Dear Syed, The diffraction pattern is a bit strange: the distant rows of very closely spaced spots point to a cell that has at least one long dimension (indicating protein), but the very strong isolated spots at mid-resolution indicate salt. Could there be two crystals on your loop by any chance? K/Na tartrate of course crystallises, but I would not expect it to at the conditions that you state, unless maybe your protein concentration is very high or the temperature very low. Could there be something else there that you are not accounting for (ethanol, some other salt left over from purification, or what have you) ? Best, Emmanuel Principal Researcher, Institute of Nanoscience & Nanotechnology National Centre for Scientific Research "DEMOKRITOS" Ag. Paraskevi, Athens 15341, Greece. tel: +30-2106503793 email: [email protected]
-----Original Message----- From: syed <[email protected]> To: CCP4BB <[email protected]> Date: Friday, 31 January 2025 8:59 AM EET Subject: [ccp4bb] Could Pottasium sodium tartarate diffract? Hi All I have a protein crystal (thin plate crystal) diffracted to high resolution. The crystallisation condition is 200mM Pottasium sodium tartarate and 20% Peg 3350. Protein molecular weight is 35 kDa. I could see the reflection spots near 1.9A. The problem is, I could not process the images. I see very low number of spots at low resolution. So I doubt whether the diffraction is from protein or from Pottasium sodium tartarate. Any suggestions, Thanks Syed To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
