Hi Firdous A good starting point is the Relion STA tutorial - https://relion.readthedocs.io/en/release-5.0/STA_tutorial/
Best regards Rangana On Fri, Apr 4, 2025 at 11:08 AM Firdous Tarique <[email protected]> wrote: > Hello everyone > > I am planning to teach a Cryo-ET course module for undergraduate students > at an introductory to intermediate level next year. The course will cover > image processing and 3D reconstruction, including tilt series alignment, > segmentation, and subtomogram averaging, aiming to achieve a resolution of > 3–5 Å for 500 kDa–2000 kDa protein complexes (e.g., ribosomes, viruses, > microtubules, RuBisCO) using raw data and tilt series obtained from the > EMDB repository. > > While I have extensive experience in single-particle reconstruction, my > knowledge of Cryo-ET is primarily theoretical. One of the key challenges is > that Cryo-ET data processing is not a straightforward, single-software > process—it requires multiple software tools for different stages, and > sometimes even multiple tools for a single step. > > Could anyone recommend a streamlined Cryo-ET workflow with a minimal set > of well-established software tools for these steps that can cover the > entire reconstruction process, from raw tilt series to high-resolution > structures? My goal is to first gain hands-on experience with the workflow > and then use it as the foundation for teaching the students next year. > > Any guidance to design this teaching module would be greatly appreciated > > Best > > Firdous > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
