Hello Lan
In PDB format your option would be insertion codes, column 27 (counting
starting at character 1, not 0), right after the residue number. If,
for example, you had a hexa-HIS after residue 22 you could call them
22A, 22B, 22C, 22D, 22E, 22F before resuming with the native protein
sequence at residue 23.
http://www.bmsc.washington.edu/CrystaLinks/man/pdb/part_62.html
Phil
On 4/30/25 2:24 PM, Guan, Lan wrote:
Dear All,
I am working on a protein structure with a His tag in the middle of the
protein. I would appreciate advice on retaining the native number in the
protein sequence after this His tag.
Best,
Lan
***********************************************************************************
Lan Guan, MD, PhD
Professor | Department of Cell Physiology and Molecular Biophysics
Co-Director | Center for Membrane Protein Research
3601 4th St. MS 6551
Texas Tech University Health Sciences Center
Lubbock, TX 79430
5A148A (Office)| (1) 806 743-3102 (Phone) | lan.g...@ttuhsc.edu (E-Mail)
https://www.ttuhsc.edu/medicine/cell-physiology-molecular-biophysics/faculty/guan/
https://www.ttuhsc.edu/centers-institutes/membrane-protein-research/
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