Dear Nikolas,
You should contact the depositing authors requesting corrections. 
I have done that several times. Usually it works and some of the
responsible groups were really happy.

If not feel free to deposite an own correction ... 

best,

Winfried Hinrichs

----------------------------------------------------------------------
Dr. rer. nat. Winfried Hinrichs
Professor and Chair of Biochemistry, Emeritus

University of Greifswald
Institute of Biochemistry
Felix-Hausdorff-Str. 4
D-17487 Greifswald, Germany

E-mail [email protected]

http://orcid.org/0000-0002-0435-4565
----------------------------------------------------------------------


Am Freitag, den 30-05-2025 um 23:30 schrieb Nikolas:


*enters the BB and kneels in reverence*


Dear BB,


Recently, delving among papers and structures for a current project, I
came across a deposited structure of interest that is terribly
modeled. In the PDB resolution is 2.00 Å (R/Rfree=0.19/0.22) and the
quality indicators are not terrible but upon opening it and checking
the maps there are some severe mistakes in the modeling.
For instance, a full chain that is in the negative Fo-Fc while
positive density is present, some density that is quite clear for SO4
molecules (buffer) modeled as water and density for water molecules
neglected. Some parts seem like “coot:runwater” has been used and
not checked. The catalytic site is well defined.


Now, I’ve started my journey in crystallography not too long ago and
I’ve got many years, structures to solve and things to learn, and I
know that sometimes the dataset “is what it is” but these seems
quite big mistakes. Especially because I’ve worked with such
proteins before and I know the systems.


My question thus is: would it be right to contact the author of this
structure and point to these issues? What’s the best way to address
such things, possibly without coming off as obnoxious?


Thank you for your wisdom.


Cheers,
Nikolas


*bows and leaves the BB*




 

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