Dear Andrea, This patent has nanobodies against different light chains, incl. for lambda isotype. I know some colleagues used the kappa one, but I recall (vaguely) from some discussion that the lambda ones were not very high affinity. But don’t take my word for it, third hand information. https://patentscope.wipo.int/search/en/WO2006059904
best, Marko From: CCP4 bulletin board <[email protected]> On Behalf Of Andrea Smith Sent: 03 February 2026 14:54 To: [email protected] Subject: [ccp4bb] Anti–human lambda light-chain binders for Fab stabilization Hi all, I’m looking for a small, high-affinity binder to the human lambda light-chain constant region—essentially a lambda-specific analog of the anti-Cκ VHHs that have been used to stabilize Fabs for cryo-EM. We have a Fab with a lambda light chain and would like a similar binder to form a stable Fab–binder complex (for cryo-EM and potentially crystallization). Ideally this would bind outside the paratope and be relatively compact. If anyone knows of existing anti-lambda binders, has used something like this successfully, or can suggest a commercial source, I’d really appreciate any advice. Thank you, Andrea Smith ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
