I am designing a bacterial protein interaction and co-localisation study involving two interacting proteins.
One protein is very small (~7–8 kDa), while the other is approximately 25–30 kDa. Previous biochemical experiments (crystal structure and binding studies) have confirmed that the two proteins interact, and one protein modulates the function of the other. My initial plan was to generate fluorescent protein fusions (EGFP and mCherry) for live-cell co-localisation. However, I am concerned that fusing a ~27–29 kDa fluorescent protein to the smaller protein may interfere with its folding, localisation, or interaction. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
