A second ejaculation an hour later contains 70% of the amount of sperm of
the first ejaculate.
L



Theriogenology 1999 Oct 15;52(6):981-6
Semen quality in dogs and the influence of a short-interval second
ejaculation.

England GC.

Department of Farm Animal and Equine Medicine and Surgery, Royal Veterinary
College, University of London, Hatfield, Herts, England.

Semen quality was examined in each of 65 known fertile dogs. Values were
found to be similar to those previously published, although an apparent
breed influence was demonstrated, with German shepherd dogs producing
ejaculates of larger volume and greater total spermatozoal output than other
breeds. A second ejaculate was collected from each dog with a mean interval
of 63 min. The second samples had significantly lower values for the volume
of the second fraction, the spermatozoal concentration and the total
spermatozoal output. There were no differences for the percentage motility
or the percentage of morphologically normal live spermatozoa. While there
was no increase in semen quality of the second ejaculate, the technique may
be useful since it results in the collection of approximately 70% more
spermatozoa than a single ejaculate. These spermatozoa also had normal
motility and morphology and could, therefore, be used for insemination or
cryopreservation.

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