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We have identified human monocytic (THP-1) and myelogenous CD34(+) (KG-1)
leukemia cell lines that can be differentiated rapidly into mature dendritic
cells (DCs) when cultured in serum-free medium containing GM-CSF, TNF-alpha, and
ionomycin. These hematopoietic cell line-derived DCs are highly pure and
monotypic, and display the morphologic, phenotypic, molecular, and functional
properties of DCs generated from human donor-derived monocytes or CD34(+)
hematopoietic progenitor cells. During differentiation into mature DCs, the
cells exhibit de novo cell-surface _expression_ of CD83, CD80, CD86, CD40, CD206,
CD209, CD120a, CD120b, and intracellular synthesis of IL-10, increase their
endocytotic capacity, and acquire characteristic stellate morphology.
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