For the record, the copy of the Refmac library that you get with a Coot
binary tar file unfortunately does not contain the CYS-BME. AFAICS, the
Refmac library that comes with CCP4 6.1 does not contain CYS-BME either.
You can build your own version of the CYS-BME using CYS-MPR (the
propionate form) as a template.
However, when you have done that, I doubt that Coot will handle the link
properly - I'll see what I can do about that.
Regards,
Paul.
Garib Murshudov wrote:
You can use a link for this. The name of the link is
CYS-BME
To make sure that you have this link in the dictionary have a look:
$CLIBD_MON/list/mon_lib_list.cif
and search for CYS-BME
If you have this in the dictionary then you can just add a link record
in your pdb file. Example how to use a link in the pdb is in:
www.ysbl.york.ac.uk/refmac/data/template_link.pdb
Garib
On 14 Jan 2009, at 08:25, Don Kerkow wrote:
Hi,
I appear to have a BME adduct on one of my cysteines and was just
wondering how I form
the disulfide bond from the Cys to my imported BME monomer? Also,
what considerations,
if any, need to be taken when depositing a structure to PDB with a
modification/adduct like
this?
Thanks for any help in this regard!
Don Kerkow
