Hi, I'm new to crystallography and am wondering when biulding a model in Coot do you use:
A) the .MTZ file that was generated from Scalepack2mtz as your map which causes Coot to run Refmac and display an electron density map -or- B) the .MTZ file that was generated after using Phaser for molecular replacement as a map without running Refmac When I used method 'A' I was able to build a good model. The same model opened with the map from method 'B' had a lot of rotamers that were not optimal. So which method is correct and why? Thanks! -JKF
