Hi Cooters, I have a pair of disulfide bonds which link two monomers in separate asymmetric units. There is a single monomer in the asymmetric unit, and two monomers come together to form disulfides between Cys 26-Cys45, and Cys45-Cys26. I'd like to use Coot to refine them, but every time I use the Real-Space Refine Zone command it sticks the S atom into the middle of the shared density, resulting in a S-S distance of half an angstrom. As much as I'd love to say this enzyme is powered by nuclear fusion, I think a reviewer would disagree. I have SSBOND lines in the PDB file for both bonds, and Refmac refines the bond lengths to an appropriate 2.03 angstroms. Is there a way to make Coot respect these bonds?
Regards, Mark
