Hi All, Very sorry for spamming,,,but I realized my mistake, and I thought I'll let you know before I take away anyone's time on this. What I ought to have done is superimpose the maps first. My mistake is that by superposing the molecule B over molecule A first, I was changing the coordinates of B (obviously) and then when I was trying to create the transformation matrix to superpose the maps, I was actually using the changed coordinates of B, and so nothing was happening. It was like an identity matrix (assuming B fit perfectly over A). I'm sorry, I didn't think this out the first time before posting the question.
Thanks, Subhangi On Fri, Jan 28, 2011 at 5:52 PM, Subhangi Ghosh <[email protected]>wrote: > Hi All, > > If, in Coot, I want to study two molecules A and B superimposed on each > other with their maps superimposed too, then I'll first have to use the LSQ > superpose to move B over A, then use the same transformation matrix on the > map of B. I do this in Coot by Extension -> Maps -> Transform map by LSQ fit > and then specify A as the fixed molecule and B as the moving molecule and > then specify that the map of B needs to be transformed. > This does not seem to be working? Is my method incorrect? Please help. > I'm using Coot -0.6.2-pre-1. > > Thanks very much. > Subhangi > > > >
