Hi everybody,
Have there been any advances or are there specific settings in COOT
for low-resolution regularization or real-space refinement, along the
lines of lego in the O program? I have found some old threads on the
topic, such as this one:
http://www.ysbl.york.ac.uk/~emsley/coot/mbox-2006/0640.html
I am having trouble using COOT, building into 3.8-4 A maps (the maps
are rather good for the resolution; 2-fold NCS, high solvent content),
for the idealization of beta-strands in B-factor sharpened maps. This
does not work well in COOT for me, the mainchain is frequently moves
outside of the density. I did reduce the matrix term to 20, but keep
having trouble.
Any suggestions are very welcome.
Cheers,
Florian
