I have to mutate 6 membered ring structure, with 2 Natoms and 4 C atoms.
We are talking about modifying a ligand, I take it?
There was no hydrogen atom on the nitrogens. after necessary mutations i have add a hydrogen atom on one of these N atoms.
Using some tool other than Coot, I presume? Or are we talking about the restraints editor?
But due to the diff in bond lengths H is not adding.
What did you do to try to add the H? What did you expect to happen? What actually happened?
So i have to slightly change one of the bond lengths to make it single bond. is there any method to do this with altering or breaking the whole rong structure.
If we are talking about modifying restraints, then changing the bond length does not change the bond order.
I am not convinced that we are talking about Coot. Using the restraints editor is the the recommended way of manipulating chemistry.
HTH, Paul.
