Hi Bobby, > I am trying to refine a protein with two ligands. One of the > ligands apparently will not allow me to do real space refinement in > coot after I import it as a cif file, created from sketcher.
It is not clear to me what reason you have for using sketcher (it is not the first tool that jumps to (my) mind to use with ligands these days). > It is a known ligand in structure deposited in the pdb as 1RQI. I see DST, IPR and DPO. The restraints for each of those are autoloaded using the standard refmac monomer library (I am using CCP4 6.3, but I would imagine that the same would happen using 6.2). Just "click click" on an atom of the residue and refinement starts. > I could not retrieve the ligand with the three letter code and the > file looks similar to the other ligand, Hmm... oh dear (if by "retieve" you mean what I would call "auto-load"). > except it contains a sulfur. I am not sure what TLCs you mean.Is coot giving you an error message? If so, what is it? Regards, Paul.
