On Mon, 2012-12-17 at 13:17 +0000, Chrislaine Withers wrote: > Hi everyone, > > I would be very grateful if you could help me with the following: > > I am refining a protein that has been treated with chymotrypsin prior to > crystallisation. As a results, there are nicks in the chain but the chain has > remained associated due to SS bonds and Ca sites. How could I model these > breaks in Coot? The program tends to reform the peptide bond whatever I do to > keep it apart... I would like to keep the same identifier for the chain and > to be able not to form bonds when required. > > Many thanks for your help. > > With regards, > > Chrislaine.
There might be more elegant way, but you can either anchor atoms to their positions or temporarily break your protein into multiple chains. Or renumber residues to introduce a gap. -- Coot verendus est
