Hi - I'm working on a mammalian derived crystal. In a few chains where I can see the N-terminus, it appears that they are acetylated. I looked for previous discussion on how to deal with this, and I was wondering how exactly to deal with this in coot as of 2013.
Thus far, I added an ala to the n-terminus, then used extensions>modelling>replace residue to mutate to ACE I'm running coot 0.7 stable release sept 2012. I'm not seeing a bond between the original N-term N atom and the C of he acetyl group Real Space Refine Zone and Regularize Zone do not like the setup. I'm sure I need to define some things, but haven't dealt with this before, as for things like this, I used to switch to xfit. Would it be better to ask at CCP4? Thanks, Dave
