-Caveat Lector-

Simple Test Allows Shoppers To Check Produce for Engineered Content
12.59 p.m. ET (1659 GMT) September 7, 1999
http://www.foxnews.com/js_index.sml?content=/news/international/
By Roger Dobson

LONDON — An instant test for GM foods could soon allow shopkeepers and
customers to check whether produce is totally free of modified crops.

One of the problems with GM products is that it is difficult to tell them
apart from natural foods. Many processed foods thought to be GM-free have
been found to contain small amounts of engineered material. It is possible
to carry out DNA tests to spot the difference in the crop gene, but that is
time-consuming, expensive and often impractical.

The new test, developed by Strategic Diagnostics of Delaware in America,
adapts the kind of antibody technology first used in DIY pregnancy kits to
track down the genetically modified crops.
The key to the success of the tests is that crops that have been
genetically engineered produce a different type of protein. A plant
engineered to be resistant to a specific insect, for instance, will express
a protein toxic to that pest. With a herbicide-resistant crop, the plant
will express a protein that de-activates the effect of the chemical.

The test developed by Strategic Diagnostics measures these proteins, which
would not be expressed by a normal plant. One test can measure the actual
amount of protein in the sample, while the other is a yes-no litmus-paper
type test that simply shows in minutes whether there is any GM food
present.
Don Durandetta, business manager for agricultural products at Strategic
Diagnostics, says that unlike DNA tests, which are looking for the gene
that creates the protein, the new test measures the protein directly and is
much faster.

He says: "The yes-no test takes only four or five minutes to give a result.
It is essentially the same format as a standard home-pregnancy kit."

The testing strip contains antibodies that are specific to the protein
being looked for. On one side is a monoclonal antibody and on the other a
polyclonal antibody. Attached to one of them is a color marker. When the
strip is immersed in the crop sample that has been mixed with water to make
it fluid, the sample flows along a line between the arrays of antibodies.

When the protein to which the antibodies react passes along the line, the
antibodies on either side close in and form a sandwich, triggering the
marker and changing the color of the strip.

"It is a simple yes-no sandwich assay test and the only way you can end up
with a colored strip is if both antibodies respond and a sandwich forms to
change the color, and that shows that there is transgenic protein present.
This particular test doesn't tell you how much there is, just whether or
not there is any present in the sample.

"The first applications are at the producer end. Our biggest market at
present is the quick yes-no test, which can screen trucks as they roll up
from the farm. You can test a truck while it is still on the scales. You
can take raw grain out of trucks as they arrive, grind it up, shake it up
with water, take a sample and drop a strip in. It takes three or four
minutes and the whole process, including gathering the sample, takes six
minutes."

The company is currently testing the effectiveness of the strips on
processed products. If found to be useful, this could lead to a consumer
version that could quickly tell shoppers if food contains any kind of
GM-modified ingredients.

One of the advantages for food producers is that the test will allow them
to guarantee that there is no GM material in their crops. For food
suppliers and shops, it means that they will be better able to put similar
guarantees on processed-food packaging.

Eventually, the antibody technology could be applied to many other forms of
GM food as they start to come onto the market.

With new GM legislation likely in Europe, Japan and America, the tests
could protect producers from legal action where food that is meant to be
GM-free is found to have a transgenic content.

It would also mean that such food would not have to be junked as was the
case in America when thousands of boxes of organic chips were destroyed
because the corn used had been accidentally contaminated by a crop from a
neighboring field of transgenic plants.

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