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From: "Ian Goddard" <[EMAIL PROTECTED]>
To: <[EMAIL PROTECTED]>
Subject: Dental Amalgam & Brain Impairment
Date: Sunday, January 21, 2001 12:25 PM

STUDY: "...we conclude that chronic inhalation of low-level Hg0
[mercury vapor from amalgam fillings] can inhibit polymerization
of brain tubulin essential for formation of microtubules."

ABSTRACT:

Neurotoxicology 1997;18(2):315-24

Mercury vapor inhalation inhibits binding of GTP to tubulin in rat
brain: similarity to a molecular lesion in Alzheimer diseased brain.

Pendergrass JC, Haley BE, Vimy MJ, Winfield SA, Lorscheider FL

College of Pharmacy, University of Kentucky, Lexington 40536, USA.

Hg2+ interacts with brain tubulin and disassembles microtubules that maintain
neurite structure. Since it is well known that Hg vapor (Hg0) is continuously
released from "silver" amalgam tooth fillings and is absorbed into brain, rats
were exposed to Hg0 4h/day for 0, 2, 7, 14 and 28 d at 250 or 300 micrograms
Hg/m3 air, concentrations present in mouth air of some humans with many amalgam
fillings. Average rat brain Hg concentrations increased significantly (11-47
fold) with duration of Hg0 exposure. By 14 d Hg0 exposure, photoaffinity
labelling on the beta-subunit of the tubulin dimer with [alpha 32P] 8N3 GTP
in brain homogenates was decreased 41-74%, upon analysis of SDS-PAGE
autoradiograms. The identical neurochemical lesion of similar or greater
magnitude is evident in Alzheimer brain homogenates from approximately 80%
of patients, when compared to human age-matched neurological controls. Total
tubulin protein levels remained relatively unchanged between Hg0 exposed rat
brains and controls, and between Alzheimer brains and controls. Since the rate
of tubulin polymerization is dependent upon binding of GTP to tubulin dimers,
we conclude that chronic inhalation of low-level Hg0 can inhibit
polymerization
of brain tubulin essential for formation of microtubules.

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