No Vaccine, No Cure - but the money boys are working on the vaccine
which will be given to little babies who would probably not get this
disease at all if raised properly and taught how to avoid same?


Saba


The Scientist 15[13]:1, Jun. 25, 2001
NEWS

No Vaccine, No Cure
HIV/AIDS: 20 Years and Counting

By Myrna Watanabe

Editor's Note: This is the second of two articles that looks at the
progression of AIDS research over the 20 years since its identification.
The first part: M.E. Watanabe, "AIDS, 20 years later," The Scientist,
15[12]:1, June 11, 2001.

Despite billions of dollars spent in research funds and a brief reprieve
in Western nations after the introduction of multidrug therapy, AIDS
continues to win its battle against humankind. First diagnosed 20 years
ago, there are still no cures and no vaccines. Prevention remains
elusive and worldwide infection numbers are skyrocketing. A vaccine is
still a decade or more away.

When first diagnosed in the United States, the disease mainly struck gay
men, intravenous drug abusers, and those who had received tainted blood
or blood products. Today, its targets have changed. AIDS thrives in
poverty and among those who are disenfranchised. Increasingly, it is
becoming a disease affecting women and children: worldwide, in 2000, 2.2
million of the 4.7 million new HIV infections were in women.1

In the United States, "it continues to disproportionately [strike]
communities of color," says Helene Gayle, the outgoing director of the
Centers for Disease Control and Prevention National Center for HIV, STD,
and TB Prevention. "Almost 70 percent of new HIV infections are among
African Americans and Latinos." Moreover, the prevalence of AIDS in gay
men in the United States is no longer declining, after years of
decreasing. "It's not enough to tell people that this is AIDS and how
you get it," says Karungari (Karusa) Kiragu, research writer and
associate, Population Reports, Johns Hopkins University Center for
Communication Programs. "What do you say to young people, and will they
listen?"

Today, the disease's epicenter is in Sub-Saharan Africa, where,
according to the Joint United Nations Programme on HIV/AIDS (UNAIDS),
25.3 million people are HIV-positive, for a prevalence rate of 8.8
percent of those between 14 and 49 years of age.2 Its spread is abetted
by cultural traditions, prostitution, lack of public health
infrastructure, governmental indifference, superstition, ignorance and
poverty. In some parts of the world, specific "cultural factors...will
put women at risk," Kiragu says.

Nearly 20 antivirals on the market today have changed AIDS from an
absolute death sentence to a potentially chronic disease, but these
drugs are very expensive and usually not available in developing
countries. "It's a pretty disturbing experience to ...leave your
patients here because they're doing great, and walk into a hospital [in
South Africa]," states Bruce Walker, director of the Partners AIDS
Research Center, Massachusetts General Hospital, Harvard Medical School,
who has been treating AIDS patients since the mid-1980s. "You know you
have medications here and [if administered] they can get up from their
death beds. Instead, they're going to die."

Only recently have pharmaceutical firms, under intense pressure from
activists and politicians, agreed to provide drugs at a reduced cost or
for free to some of the developing nations. A consensus statement,
spearheaded by Harvard University economist Jeffrey Sachs, estimates
that HIV testing, drug treatment, clinical follow-up, and research on
HIV in Africa would cost $1,123 per patient per year.3

But distributing drugs and following up on patients are problems. "Even
if you parachute [in] all the drugs that you need for a developing
country, you are still going to have great trouble distributing them
because the health care infrastructure in these countries is nonexistent
or minimal," says Anthony Fauci, director of the National Institute of
Allergy and Infectious Diseases (NIAID). "This might be an opportunity
to jump-start interest in developing this health care infrastructure in
these developing nations." Harvard's Walker sees more opportunity: "Once
you have the clinic with the health care staff that can deal with the
HIV infection, they can deal with other infections as well."

Even when the drugs get distributed, current pharmaceutical treatment
does not cure HIV/AIDS. "We need a new set of drugs," states Gregg
Gonsalves, an activist with the Gay Men's Health Crisis in New York and
founder of the Treatment Action Group. The drugs in use are highly
active antiretroviral therapy (HAART), the multidrug cocktails that keep
those infected alive and their viral loads low or negligible. Gonsalves
notes, "All our drugs are reverse transcriptase inhibitors and protease
inhibitors. We need drugs directed at other viral targets." The HIV
virus replicates rapidly and mutates quickly. "Breakthrough" viruses
evolve and elude antiretroviral treatment--as well as the immune
systems' scavenging effects.

Furthermore, these drugs are toxic. "You run out of options and you run
into toxicities," remarks Gonsalves.

Finding a Cure

There is one therapeutic success that is saving lives in developing
nations. Susie Zeegen, cofounder of the Elizabeth Glaser Pediatric AIDS
Foundation, explains that the drug nevirapine cuts mother-to-child
transmission almost in half if given to the mother at delivery and to
the child in 72 hours. Zidovudine (AZT) also can prevent vertical
transmission from mother to fetus, but the dosing regimen is more
complex. Virologist John Moore, of the Joan and Sanford I. Weill Medical
College of Cornell University in New York, thinks the future will bring
more effective drugs with fewer side effects. Among these are inhibitors
that will prevent HIV from attaching to or entering its target cells--T
cells or macrophages. Moore and his colleagues are studying these entry
inhibitors, which he sees as "moving toward the clinic."

Early on, some researchers thought that developing an HIV/AIDS vaccine
would not be a unique challenge. AIDS vaccine researcher Margaret Liu,
senior adviser in vaccinology for the Bill and Melinda Gates Foundation,
says that researchers used the hepatitis B vaccine as a paradigm for a
putative HIV vaccine "and they started off making a vaccine before they
knew the pathophysiology of the virus. Then it became clear that it was
a different ball game, a different challenge."

Robert Gallo, then at the National Cancer Institute and now director of
the University of Maryland Institute of Human Virology in Baltimore,
notes, "there was no antiviral success.... I thought it would be
exceedingly difficult to get new therapy against HIV; [I] thought [it
would be easier] to get a vaccine."

He admits he was wrong. Another reason there is no vaccine is that, for
many years, vaccines were a stepchild of AIDS research and were
considered secondary to pharmaceutical development: Jon Cohen, Science's
HIV/AIDS correspondent, posits in his recent book that a lack of
organization and leadership has hindered the vaccine effort.4,5
Liu says that the vaccine industry had an early interest in developing
AIDS vaccines. "If you go back and look in the '80s and early '90s,
there were companies that invested quite heavily."

Among these were Genentech and its spinoff, VaxGen; Pasteur-M�rieux
Connaught [now Aventis]; Chiron; and Merck & Co. Liu worked on HIV
vaccines for Merck and then Chiron. In the mid-1980s, says Liu, efforts
to produce a vaccine started dropping off. Part of this was due to the
science. "There were still many people who clung to the paradigm of
making an antibody-based vaccine hoping to generate sterilizing immunity
as the only way to make a vaccine," she recalls. Without sterilizing
immunity, Liu says, an individual could suffer from a lifelong infection
or an infection that would be difficult to annihilate. But Liu reasoned
that an HIV vaccine that would result in a cellular response (production
of cytotoxic T lymphocytes (CTLs) rather than an antibody response)
"could target more conserved proteins, could have a vaccine that was
more widely effective." She recalls, "I found a lot of resistance [from
other researchers] when I started making an HIV vaccine that would
either be based on or [cause] a cellular response."

Sterilizing immunity may be desirable, but it may not be essential for a
useful vaccine.
Only one vaccine is in large Phase III clinical trials: VaxGen's gp120
vaccine, which contains a piece of the HIV protein envelope to stimulate
antibody production. Some researchers expect the vaccine will be
ineffective in protecting against HIV infection.6 At least a dozen
vaccine candidates are now in early clinical trials, and many more in
preclinical development.7 Among these are a series of DNA-based vaccines
used in monkey tests by Merck scientists. Two of these constructs have
protected the monkeys, challenged with a potent simian-HIV (SHIV)
pathogen--a hybrid between HIV and SIV--from high viral loads.8

Another is in monkey trials at Yerkes Regional Primate Research Center
at Emory University in Atlanta. The construct, according to lead
researcher Harriet Robinson, contains DNA plus a recombinant modified
vaccinia Ankara (rMVA) booster. The researchers allowed seven months to
elapse after vaccination with a rMVA booster, to allow long-term immune
responses to develop and then gave the monkeys a mucosal challenge with
a very pathogenic SHIV construct.9 When The Scientist spoke with
Robinson, the experiment was at 42 weeks post-challenge. All of the
controls died, but 23 of 24 vaccinated animals "are doing incredibly
well," says Robinson.

"We're going ahead now to develop the components that we think will
develop a worldwide vaccine." If this trial remains successful, the
group plans to go into a Phase I clinical trial in humans in 2002.

Courtesy of International AIDS Vaccine Initiative
Seth Berkley

These vaccines will not prevent infection, but they will stimulate
immune responses once infection has occurred, allowing the affected
individual to ward off full-blown AIDS, and perhaps even allowing them
to clear the virus from their bodies. Seth Berkley, president of the New
York-based International AIDS Vaccine Initiative (IAVI), a nonprofit
organization that is working to bring HIV vaccines to less-developed
countries, says that it's a long road to a vaccine. "We've only got one
vaccine in Phase III trials. At best, by 2003, we can have a second
candidate enter." The next two candidates would not be ready for
clinical trials until 2005 or 2006. "Right now, there's no evidence of
any vaccine that will be effective in a human population," says Ronald
Desrosiers, of the New England Regional Primate Research Center, Harvard
Medical School.
Prevention, Prevention, Prevention
Until a vaccine or complete cure becomes available, the best investments
will be in continued research and in prevention. Fauci notes that NIH's
AIDS research budget for 2002 is $2.5 billion--more than NIH has
budgeted for any other disease. Prevention encompasses education, condom
distribution, needle distribution (for intravenous drug users), and
development of vaginal or rectal microbicides.

But education must take culture into account. Wife inheritance is
prevalent in Eastern and Southern Africa, where a brother-in-law
inherits his brother's widow as his wife. "The problem is, if the new
husband has HIV, the woman may not know and may not have a choice
anyway," says Johns Hopkins' Kiragu. Another is male sexual
expectations. "Men are socialized to expect sex from women.... Women are
socialized to give in," she notes. Nor do males necessarily see condom
use as their responsibility: "If women demand condoms to be used, [it]
means they must have been running around."
Phyllis Piotrow, director of the Center for Communication Programs,
Johns Hopkins University Bloomberg School of Public Health, suggests
that education cannot be directed just at the individual. "It's also
necessary to educate the community about the ways in which
community...norms can be changed in order to support individuals who are
trying to change their behavior." She continues, "There can be a lot
more emphasis on income-producing activities for girls so that they
don't engage in survival sex to earn money .... We need to put more
focus on male behavior and to identify what is unacceptable male
behavior." Says IAVI's Berkley: "[AIDS] is the greatest public health
challenge of our time, of the century, and we are not approaching it
with the seriousness that it needs."
Myrna E. Watanabe is a freelance writer in Patterson, New York.
References
1. UNAIDS/WHO, "AIDS epidemic update: December 2000," December 2000.
2. S.G. Stolberg, "In AIDS war, new weapons and new victims," The New
York Times, June 3, 2001:1.
3. The Harvard Consensus Statement on Anti-Retroviral Treatment for AIDS
in Poor Countries is available at
www.aids.harvard.edu/overview/news_events/events/ consensus.html
4. M. Watanabe, "Science, policy issues put AIDS vaccine on slow track,"
The Scientist, 11[22]:1, Nov. 10, 1997.
5. J. Cohen, Shots in the Dark: The Wayward Search for an AIDS Vaccine,
New York: W.W. Norton & Co., 2001.
6. R.A. Weiss, "Gulliver's travels in HIVland," Nature, 410:963�7,
April 19, 2001.
7. AIDS Vaccine Advocacy Coalition, 6 Years and Counting: Can a Shifting
Landscape Accelerate an AIDS Vaccine? Washington, DC: AVAC, 2001.
8. J. Cohen, "Merck reemerges with a bold AIDS vaccine effort," Science,
292:24�5, April 6, 2001.
9. R.R. Amara et al., "Control of a mucosal challenge and prevention of
AIDS by a multiprotein DNA/MVA vaccine," Science, 292:69�74, April 6,
2001.
Collateral Knowledge from AIDS Research
Courtesy of Cetners for Disease Control and Prevention
Helene Gayle
Nothing has stopped the AIDS epidemic, but in these 20 years, society
has learned more about other diseases and more about dealing with public
health issues and with affected populations. "In two short decades, I
think we have witnessed the evolution of a disease that, in many ways,
will define how we do public health and has changed the way we conceive
of issues beyond public health," says Helene Gayle, outgoing director of
the Centers for Disease Control and Prevention National Center for HIV,
STD, and TB Prevention.
Early involvement by activist groups shaped not only the research
tenor--with pressure for drugs to cure the sick rather than vaccines to
prevent transmission--but also molded how society views drug
development. "I think that without the HIV epidemic and the involvement
of the activists, we would not be where we are in drug development in
general," remarks Arthur Ammann, founder of Global Strategies for HIV
Prevention in San Rafael, Calif. "The activists got involved and asked,
'Why can't we do research faster? Why does it take so long to get drugs
to the public?' [The movement] said that the consumer has the right to
demand accountability in every aspect of their health."
Robert Gallo, director, University of Maryland Institute of Human
Virology in Baltimore, admits that during the epidemic's early days,
after great scientific advances developed rapidly, he didn't quite
understand what the AIDS patients, primarily homosexuals, wanted. He
does now. "I would say, number one, there's a lot more understanding
between scientists, health care workers, and patients in the so-called
risk groups than there was by orders of magnitude [in] the early days. I
didn't understand what it meant to be surrounded by your friends who
were dying [who felt]: 'Why wasn't there something that gave one iota of
help to me and my friends?'"
Working together--governments, nongovernmental organizations,
foundations--is a new paradigm in treating a disease. "Private,
nongovernmental organizations have played an ever-larger role, and I
think it has nourished and strengthened the environment in which the
private sector has been much stronger, much more active, than they were
before; and in many cases, they are leading the government, showing the
way," says Phyllis Piotrow, professor and director, Center for
Communication Programs, Johns Hopkins University, Bloomberg School of
Public Health.
Bruce Walker, director of Partners AIDS Research Center, Massachusetts
General Hospital, Harvard Medical School, sees the lessons learned over
the past 20 years as a means of moving medical care in the developing
world to a higher standard. "Clearly, what we're going to see over the
next five years is finally an attempt to address some of the global
inequalities in health care delivery. My hope is that we'll use some of
the opportunities afforded by the need to treat the HIV epidemic as a
means to treat other diseases as well." Seth Berkley, president of the
International AIDS Vaccine Initiative in New York, sees a way to develop
a distribution network for vaccines. "For example, let's take hepatitis
B vaccine and create a campaign to reach adolescents and high-risk
people, who we currently have no mechanism to target .... Using
hepatitis B, we can figure out the mechanisms, efficiency, and
cost-effectiveness of different modes of delivery."
Bringing young scientists to the HIV/AIDS research field has also been
an important goal. By giving grants to researchers, especially those in
the early stages of work in immunology, virology, or clinical research,
the Elizabeth Glazer Pediatric AIDS Foundation says it has "done a lot
to try to bring new interest in this area," remarks co-founder Susie
Zeegen. "And I think we've been successful."
And there are the scientific gains. "New fundamental mechanisms in
molecular biology have come out of basic HIV/AIDS research," notes
Gallo. Says Walker: "The payoff is not only understanding HIV, but ...
other viruses and cancer."
--Myrna Watanabe
The Scientist 15[13]:1, Jun. 25, 2001
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