Hi Aaron, Aaron M. Ucko, on 2020-06-09 10:32:20 -0400: > Andreas Tille <ti...@debian.org> writes: > > > On Tue, Jun 09, 2020 at 02:25:58PM +0200, Étienne Mollier wrote: > >> My current impression is that makeblastdb is unable to work > >> properly on most 32 bits machines, because the amount of memory > >> needing to be addressed by the process looks like it might > >> exceed too easily 32 bits architectural limits. > > That's entirely plausible; upstream tends to assume 64-bit systems > nowadays. Explicitly supplying -blastdb_version 4 (the default prior to > BLAST+ 2.10.x) may help.
It looks good on my eeepc: # makeblastdb -blastdb_version 4 -dbtype nucl \ -in GenBank/NC_005816.fna -parse_seqids -hash_index \ -max_file_sz 20MB -taxid 10 Building a new DB, current time: 06/09/2020 17:20:11 New DB name: /tmp/python-biopython/Tests/GenBank/NC_005816.fna New DB title: GenBank/NC_005816.fna Sequence type: Nucleotide Keep MBits: T Maximum file size: 20000000B Adding sequences from FASTA; added 1 sequences in 0.367298 seconds. # _ And no need for PAE. :) Thank you for the workaround! Thanks Andreas for the forward! Let's squash that bug. Cheers everyone, -- Étienne Mollier <etienne.moll...@mailoo.org> Fingerprint: 5ab1 4edf 63bb ccff 8b54 2fa9 59da 56fe fff3 882d Help find cures against the Covid-19 ! Give CPU cycles: * Rosetta@home: https://boinc.bakerlab.org/rosetta/ * Folding@home: https://foldingathome.org/