Hi! One can use pubmed nucleotide easily for searching DNA sequences for a particular gene. However is there any website / debian software which also shows other information such as intron/exon boundaries, beginning / end of transcriptions? For aa sequences, to show the boundries of exons.
Also, is there any Debian program which can automate design of primers for different purposes? e.g. PCR of genomic DNA: primers in intron RT-PCR: primers must bind to cDNA only, therefore one or both primers spans across the boundries of two exons. Also, amplification regions must be as close to poly-A tail as possible after following the previous rule, so that if cDNA breaks, the breaks are less likely to be within the amplified region Thanks for your help! Mark
