Hi,
I'm about to update the epcr package but its homepage[1] says:
The e-PCR suite of tools has been retired. Please consider using
PrimerBLAST[2] as an alternative if you are interested in designing
PCR primers or identifying target sequences for your primers.
For more information, see "Electronic-PCR (e-PCR) is retiring, use
Primer-BLAST instead"[3] at the NCBI Insights blog.
Please excuse my ignorance but I know nothing about primer design at
all. I somehow suspect that the command line tool e-PCR should now be
replaced by a web tool (or am I missing something? I can not find
something inside the blast package which looks like a command line
replacement). Currently epcr has a popcon which is somehow "medium" in
our Debian Med package set - so there is some relevant user base that
is using a discontinued / replaced tool.
What do you think?
Kind regards
Andreas.
[1] http://www.ncbi.nlm.nih.gov/tools/epcr/
[2] https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi
[3]
https://ncbiinsights.ncbi.nlm.nih.gov/2017/06/28/e-pcr-is-retiring-use-primer-blast
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