Kathleen, Another way to do epidermal peels that I found even better than clear nail polish was to use Superglue. Coat then let dry and peel off the same way with tape. OR what I did was just place the Superglue on the leaf then stuck it to the microscope slide, and I peeled the top side of the leaf off when it had dried. This will leave your stomata stuck to the slide for observation. I found it easier and less time consuming than the nail polish...less steps if you already have it stuck to the slide. And to me, it was easier than messing with the tape. Good luck, Doreen
-----Original Message----- From: Ecological Society of America: grants, jobs, news [mailto:[EMAIL PROTECTED] On Behalf Of Liane Cochran-Stafira Sent: Wednesday, April 04, 2007 12:49 PM To: [email protected] Subject: Re: Epidermal peels for Fraxinus papillae Kathleen, For stomata counts we use clear nail polish/sealer. A nice thick coat, let dry, apply clear packing tape, and peel; place tape on microscope slide. Liane At 11:43 AM 4/4/2007, Kathleen Knight wrote: >1. Do you have any experience with epidermal peels? If so, what kind of >adhesive do you use? If we press and dry the leaves this summer, will we be >able to do the peels and see the papillae this winter? > *************************** Liane Cochran-Stafira, Ph.D. Associate Professor Department of Biology Saint Xavier University 3700 West 103rd Street Chicago, Illinois 60655 phone: 773-298-3514 fax: 773-298-3536 email: [EMAIL PROTECTED] http://faculty.sxu.edu/~cochran/
