Dear friends that work on spatial Ecology,

    I am proceeding with the analysis of a dataset on the spatial structure of 
canopy openness in the southern Brazilian mixed conifer-hardwood forests, and 
would like to ask your opinion on a rather simple matter on which I have doubts.

    I have six one-hectare plots subdivided in 100 10 x 10 m plots each. In the 
centre of each subplot we took a hemispherical photograh and estimated canopy 
openness. In Legendre and Fortin (1989) it is said that before examining each 
significant value in a correlogram, we must first perfom a global test, since 
several tests are done at the same time, for a given overall significance 
level. The global test is made by checking whether the correlogram contains at 
least one value which is significant after a Bonferroni correction.

    So I ask:

1)    When we have more than one correlogram (say, six) between Moran's I and 
distance classes, should we adjust the significance level to account for the 
fact that we are performing not only, e.g., ten significance tests within each 
correlogram (which are the distance classes), but also more than one 
correlogram? If yes, then the Bonferroni correction should be the significance 
level divided by which value? 60 (6 correlograms x 10 classes within each 
correlogram)? I guess this means that if we have a really large data set almost 
nothing would be significant!

       I could not find this particular information in Dale et al. (2002) nor 
in Legendre and Legendre (1998), although I suspect that it is present in this 
last reference, because it is rather large and my search has not been 
exhaustive.

2)   One of the six plots has been hit by a microtornado a few years ago, which 
damaged a rather small area, 0,25 ha. So in this area we have only 25 10 x 10 m 
plots. Again, according with Legendre and Fortin (1989) I should not perform 
the spatial autocorrelation analysis mentioned above, because there would be 
too few pairs of localities, not enough to produce significant results. I ask: 
is this true even if we divide the distance classes in a irregular manner, so 
that they have equal frequencies of numbers of pairs of points? If this would 
allow "to compute valid coefficients even in the right-hand part of the 
correlogram", it may allow valid coefficients in the middle of a correlogram 
with fewer classes?

     Dear friends, thank you very much for your attention!

     Sincerely,
 
     Alexandre

Dr. Alexandre F. Souza 
Programa de Pós-Graduação em Biologia: Diversidade e Manejo da Vida Silvestre
Universidade do Vale do Rio dos Sinos (UNISINOS)
Av. UNISINOS 950 - C.P. 275, São Leopoldo 93022-000, RS  - Brasil
Telefone: (051)3590-8477 ramal 1263
Skype: alexfadigas
[EMAIL PROTECTED]
http://www.unisinos.br/laboratorios/lecopop


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