I know rarefaction is used when you have different sample sizes. I have a peculiar situation where I have 3 communities. I have same plot sizes for 2 communities, but have smaller plot size for the third one. Now I have more number of species and individuals in the community in the third community in which I used smaller size plots.
Should I use rarefaction in this case? I am asking this because it assumes that bigger sampling size /effort would yield more species. But in my case, it is the opposite. Smaller sample size has more species than the larger size. I am confused whether to use rarefaction in this case? Can anybody put some light on this? Thanks Shrinidhi
