Hi All, I'm working on my MS, concerning the concentration and distribution of various dioxygenase genes in soil bacteria- basically, bacteria that break down really persistent pollutants.
One of the new-ish degradation pathways in this field is phn, and i'm trying to quantify its copy numbers in soil using real-time PCR. With that in mind, does anyone out there have an actual protocol for this yet? If not, how about a plasmid carrying phnAc? If not that, how about a culture of the strain it was isolated from, Burkholderia RP007? While the gene has been detected in environmental probes around here, nobody seems to have the various components for a real time PCR protocol put together, and I was wondering if anyone had some of the pieces floating around out there. - I'm trying to avoid the delay and expense of having to get myself a culture shipped in from New Zealand ;) Thank you, Inigo Howlett UT Chattanooga _________________________________________________________________ Tease your brain--play Clink! Win cool prizes! http://club.live.com/clink.aspx?icid=clink_hotmailtextlink2
