Dear List: I am interested in incorporating fluorescent dUTP into a PCR reaction in order to perform genotype screening for polymorphic loci. I have a protocol for this using rhodamine green (Molecular Probes), but this product is no longer available. Possible replacements include ChromaTide Alex Fluor 488-5- dUTP 1 mM in TE buffer (Invitrogen, catalogue number: C-11397) or Fluorescein-aha-dUTP (Invitrogen, catalogue number: F32767).
Has anyone tried either of these for this application? If so what concentrations were used in the PCR reactions? Anything else I should know? Thanks! Steve
