Hi all I am requesting your help related to analysis of SOD, lipid peroxidation and protein carbonyl assay to metamorphed froglets. My problem is that I have samples of entire frogs frozzen under -80°C and the SOD and lipid peroxidation require to homogenate the frogs using different buffers to run the analysis. Moreover the protein assay kit analyses mention that samples containing significant haemoglobin may interfere with the assay.
I will really appreciate any suggestion and advice on how to proceed without compromise the results of the analysis. Thanks Eric
