Hello fellow Ecologgers: I'm about to embark on a SNP genotyping project and the final two platforms I'm considering are Sequenom and Biomark by Fluidigm. If anyone has used both or is quite familiar with the platforms (especially with projects facing low target copy number and mixed genomic samples), I'd really appreciate advice:
Fluidigm's reaction volumes are on the nanoscale and thus most protocols recommend preamplification of the target loci. This seems to address a major concern of mine in that my templates will have low target copy number. It is largely for this reason I'm leaning toward Fluidigm rather than Sequenom. On the other hand, Sequenom is well used and has been around for quite some time; I take comfort in tried-and-true genetic techniques. The cost to execute my research is fairly similar between the two, but I cannot afford the time or resources to test both platforms (although, that would be pretty helpful!). Please respond to: [email protected] Thanks, Katy -- Katy L Richards-Hrdlicka, Doctoral Candidate Yale University, School of Forestry and Environmental Studies 170 Whitney Ave, ESC Room 158 New Haven, CT 06520 http://environment.yale.edu/profile/kathryn-richards-hrdlicka
