Making slides of tardigrades Dear Ecolog-Listers:
I am helping a student who is working on tardigrades. The student wants to prepare slides of the active form of tardigrades (ideally permanently). Thus far, we are finding Hoyer's mounting medium as the most common liquid but the problem there is twofold: getting the chloral hydrate and the slides are not really "permanent" (at least in my experience). Someone else has used polyvinyl-lactophenol (I have no experience with this medium. Questions: 1. Can anyone direct me to best practices for making (ideally) permanent slides of tardigrades? 2. Another protocol calls for "ringing" the prepared slides using Glyptol. I have plenty of experience with Glyptal. I am not sure if Glyptol is as good as I think Glyptal was. Will appreciate constructive replies directly to: [email protected] With gratefulness, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
