Hello,
For a research project, we need to preserve some insect eggs in
RNAlater. These are soft eggs about 2 mm long and 1 mm diameter.
Some questions:
1) do you have a suggested protocol? (we need to ship them to another
lab while protecting the RNA)
2) Should we homogenize them or not?
3) Should we freeze the eggs (homogenized or not) IN the RNAlater, or
remove the RNALater before freezing (if homogenizatin not recommended)?
4) Generally in what order should we do steps (freeze first then
RNAlater? or freeze IN RNAlater?... or something else entirely?).
Thanks!
ATD of ATB and ISI
--
Aaron T. Dossey, Ph.D.
Biochemistry and Molecular Biology
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