First, I am a molecular biologist and have only taken intro stats
courses during my undergrad days.
I am trying to find a criterion to use as an objective cutoff to discard
data from experiments. I use non-linear regression curvefitting, and
Prism, so I emailed graphpad tech spt. Here is the email, and response:
---------------------------------
I have not heard of the term lambda used in that context. I don't know how
to help you in this regard. Sorry.
-----Original Message-----
From: Koichi Kunitake [mailto:[EMAIL PROTECTED]]
Sent: Friday, December 03, 1999 6:48 AM
To: [EMAIL PROTECTED]
Subject: Prism, fitting question
Hello, I am running Graphpad Prism, 2.0 (mac) at the Salk Institute for
Biological Studies. I am analyzing radioligand binding assays with one-site
competition, and am trying to determine a criterion for when I should throw
out a bad assay. I was talking to my boss, and he suggested that I use
"lambda". I looked all around for a definition of this term, and finally
found it in Statview:
"Lambda is a quantity that is used to compute power. It is sometimes called
"partial eta squared" or noncentrality value," because the power value comes
from a computation of the noncentral F distribution, based in part on lambda
and indirectly on alpha."
Does graphpad recommend using "lambda", or are there better criteria for my
purposes. If you recommend another test, what is it, and why? Also, is there
any way to make Prism calculate "lambda"?
Thanks for any help,
Koichi Kunitake
--------------------------------------
If anyone has any information how to compute "lambda", I would greatly
appreciate it (in case it helps, my boss was referring to the antiquated
(1975) program, BIOPROG, which gives "lambda" values.)
Thank you,
Koichi Kunitake
<[EMAIL PROTECTED]>
