Hi
We are using Pepstat for molecular weight calculations and subsequent
comparison with mass spectrometric determined masses.
I am looking for the mass table used for the molecular weight calculations of
the proteins in order to determine the accuracy. And how it could be possible
to change it.
The other question is implementation of a molecular weight assuming that the
cysteins form disulfide bridges.
This question is related to my first line. Since
we compare the intact molecular weight of the proteins we want to be as precise
as possible and thus measure the difference between reduced and oxidized
cystein residues. Most proteins with cystein residues form disulfide bridges.
Would it be possible to include a molecular weight
calculation which takes disulfide bridges into account? So that an even nr of
cysteins are calculated with the mass of oxidized cysteins (S-S) and if there
should be an single cystein left then it is calculated with a sulfhydryl group
(SH)?
Best Regards
Carsten P. Sönksen
Senior Scientist
Novozymes A/S
Krogshoejvej 36
2880 Bagsvaerd Denmark
Phone: +45 44461123
Mobile: +45 30771123
E-mail: [email protected]
Novozymes A/S (reg. no.: 10007127). Registered address: Krogshoejvej 36 DK-2880
Bagsvaerd, Denmark
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