I discovered that the programs using restriction enzymes do not work on my installations of EMBOSS:

ERROR   application terminated
   EMBOSS An error in remap.c at line 236:
Cannot locate enzyme file. Run REBASEEXTRACT

So I tried to follow the instructions on the EMBOSS site at sourceforge:


OK, woken from the nightmare, thanks to Guy and Mahmut. Always forget that even though I'm an admin user at the GUI level on my Mac I need to sudo to install things on the terminal.

(In my defence, if I hadn't been asked for files that weren't mentioned in the docs I might have considered that it was my fault rather than emboss's.)

David

___________________________________________________

Dr. David P. Leader, Faculty of Biomedical & Life Sciences,
University of Glasgow, Glasgow G12 8QQ, UK
Phone: +44 (0)141 330 5905
http://doolittle.ibls.gla.ac.uk/leader
http://motif.gla.ac.uk/

The University of Glasgow, charity number SC004401
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