oh no ... i need some help again ... my colleagues tell me (similar to the issue resolved last week) peptstats and protparam calculate different pIs.
i could not find in the pepstats docu, how it calculates the pI. the file eamino.dat does not seem to contain any pKas, and i could not identify any other file by name that would contain pkas. i any case, my feeling is i would have to manipulate some input to feed pepstats the values from *Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions* Electrophoresis Volume 15, Issue 1, Date: 1994, Pages: 529-539 Bengt Bjellqvist, Bodil Basse, Eydfinnur Olsen, Julio E. Celis since this seems to be what protparam uses. can anybody help ? alternatively, of course, if i can convince my team pepstats values are better then protparam values, that would be fine, too ... ingo
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