I replaced the eprimer3.c from EMBOSS 6.3.1, complied and, worked! Thank
you for the help.
It is a tricky decision. By now, I suggest adding a warning in the
eprimer3 manual page?
Regards,
Felipe
El 02/10/10 19:22, [email protected] escribió:
Hello Felipe,
Thanks for the reminder of this. Downloading the developer's version
will not help.
A version of eprimer3 that should work with primer3 2.x.x can
be downloaded from:
http://www.ebi.ac.uk/~ajb/eprimer3/eprimer3.c
if you're desperate. Note that it hasn't been tested very recently and is
not, as yet, official. We are still debating how to deal with
new and old versions of primer3. An odd hindrance
is, given that it would be useful to keep the
1.x.x version around for compatibility reasons, what to call them.
If you choose to try the above then any feedback would be welcome.
ATB
Alan
Dear EMBOSS developers and users,
I am having some problems with the eprimer3 application in EMBOSS. I
have been using primer3 online (primer3plus, ncbi site) with good
results, and I just tried using it in EMBOSS. I am using MacOS 10.6.4
(Snow Leopard), with EMBOSS 6.3.1 installed by Fink (an UNIX porting
system for MacOS). The primer3 version is 2.2.3.
One of the problems has already being described in this list, but it
seems to still be around. When I call eprimer3 with a sequence, the
output is:
Picks PCR primers and hybridization oligos
Error: Missing SEQUENCE tag
See this thread for details, I did the same tests and the results were
similar; primer3_core is OK, but it seems that eprimer3 has not been
updated yet to successfully communicate with the primer3_core 2.x
versions:
http://www.mail-archive.com/[email protected]/msg01405.html
I have not tried yet the developer's version of EMBOSS, should I?
Regards,
--
--------------------oOo--------------------
Felipe MartÃnez-Pastor, Ph. D.
ITRA-ULE (Research in Assisted Reproductive Technologies)
INDEGSAL (edificio Biomedicina)
University of León
Campus de Vegazana
24071-León (Spain)
Office phone: +34 987 291 000 + 5679
Fax: +34 987 291 282
Mobile: +34 687 365 362
e-mail: [email protected]
[email protected]
website: http://itra.unileon.es
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