Paul,
You might want to have a look at section 5 of the primer3 documentation
("CHANGES FROM VERSION 2.2.3"). They changed the default for
PRIMER_THERMODYNAMIC_ALIGNMENT from 0 to 1. If this is left at 1, then you
also need to supply a value for PRIMER_THERMODYNAMIC_PARAMETERS_PATH.
You can revert to the old defaults using this command-line argument.
--default_version=1
Scott
Scott Markel, Ph.D.
Principal Bioinformatics Architect email: [email protected]
Accelrys (Pipeline Pilot R&D) mobile: +1 858 205 3653
10188 Telesis Court, Suite 100 voice: +1 858 799 5603
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-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Paul Tanger
Sent: Thursday, 13 September 13 2012 1:25 PM
To: [email protected]
Subject: [EMBOSS] specify primer3 directory?
Hi,
I have a local install of emboss and I'm trying to get eprimer3 to
work, but I'm getting this error:
"Error: thermodynamic approach chosen, but path to thermodynamic
parameters not specified"
primer3 is installed, but not in the default location (which I think
is /opt/primer3_config ?) .
How do I specify where primer3 is installed? Or is the cause of this
error something else?
googled for an answer for a while, but couldn't find one.
Thanks!
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