In the mean time I managed to solve myself the problem, the hard way, by looking into the source code of primer itself. Seems that the current version needs thermodynamic parameters and searches the files that contain them either in the working directory or in /opt, unless you set a parameter to tell otherwise. I changed the source code of the EMBOSS program eprimer32, so as to add : eprimer32_send_stringC(stream, "PRIMER_THERMODYNAMIC_PARAMETERS_PATH", "/OPT/primer3-2.3.5/src/primer3_config/"); I could of course instead have copied the primer3_config directory to /opt but I preferred to do it this way.

Regards,
Guy Bottu


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