The 2008 retrovirus document can be downloaded here:

As for breaking the tie, your vet could run a confirmatory ELISA using a 
different kit and plasma or serum rather than whole blood. But really, if 45 
days is what they want, that works. 

The FeLV testing protocol suggested on is:

For healthy cats:

For sick cats:

On Jun 13, 2013, at 1:52 AM, Karen Harshbarger <> 

> All the younger ones are 13 months old----are from the same litter and there 
> is one senior.  The female never did test positive (and she lived with them 
> at the start of her life).  I think the first one didn't make it because we 
> did not realize what we were dealing with and were not aggressive enough.  
> They had all been sick with upper respiratory and couldn't seem to get rid of 
> it and it just kept passing it around.  If we had only known just how serious 
> it was and that they had been exposed to leukemia somewhere along the line 
> (not sure exactly how) we would have moved them into the garage sooner and 
> been much more aggressive (and then Tig would have made it maybe---that is 
> what I keep kicking myself about).   Once we realized what we were dealing 
> with we got major aggressive on everything--one sneeze or one little running 
> of the eye, or sleeping too much, or not cleaning their food dish, etc and 
> off to the vet we went. It scares me to think how much of our retirement 
> savings we have spent---but I tried and tried to find financial assistance 
> and just could not, so what do you do---couldn't let any more of these babies 
> just waste away like Tig did if I could possibly do anything about it---so we 
> just kept trying and are still treating aggressively even after the negative 
> test.   We also changed their diet from the cheapest we were using cause we 
> had so many cats to a better more expensive food. 
>  But we are not out of the woods yet.  The way my vet and some of the reading 
>  I have done explains that you need to get two tests that say the same thing 
> because sometimes the test is wrong.  Since 4 of them had a positive test and 
> then a negative test, we need the third test to break the tie so to 
> speak----hopefully the 3rd test will be negative and then my vet said we can 
> rest assured that they are truly negative.  The little female tested negative 
> twice, so we keep her separate and figure she is pretty much in the 
> clear---at least hope.   (the two little ones that the no kill rescue took 
> for us are about 10 months old by now and as I said  one tested positive and 
> one tested negative---we don't know how they came out on the second test but 
> were told a few months back that they were both doing good).
> I would be interested in that research paper that you speak of.  We all need 
> to do as much as we can to fight and learn about this horrible illness.  
> thanks Karen

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