Re: from Dr. Susan RE: PCR test and FeLV answer

[anzajaguar]

In a message dated 1/31/2005 9:26:04 P.M. Eastern Standard Time, [EMAIL PROTECTED] writes: It is a mistake to assume that a new technique such as PCR is more accurate than exisiting methods until that has been confirmed. It turned out not to be more accurate when done by commercial labs for diagnosis of FIV, and work done at the University of Guelph suggests that PCR for FeLV may not be any better in reply. from ANTECHS site (http://www.antechdiagnostics.com/clients/antechNews/2004/sep04_01.htm ) >>The polymerase chain reaction (PCR) test has been used for the diagnosis of FeLV infection. This test detects viral nucleic acid sequences instead of protein antigens. PCR may be useful in helping to determine the true status of cats with discordant results from other testing techniques. PCR is capable of detecting FeLV infection in blood, solid tissues, tissue cultures, and fixed specimens. also ANTECH refer's to "screening tests" as the ELISA test   >>Although testing is relatively straight forward, no test is accurate 100% of the time. It is possible to have discordant test results, especially if the tests target different disease stages or require different specimens. In populations with a low prevalence of infection, >50% of cats with positive test results may be uninfected. Since the consequences of both false-positive and false-negative test results can be disastrous for individual cats or for multiple cat populations, confirming positive test results is crucial, especially in asymptomatic cats. Generally, screening tests are believed to be more sensitive for FeLV and FIV infection (fewer false-negatives), whereas the advanced confirma-tory tests are felt to be more specific (fewer false-positives). It is actually a very informative and interesting article considering our recent discussions on the different types of test....   and yes it can be easily contaminated..I stated that and also "when done correctly" was in my post regarding PCR testing.  It is too expensivee for most people to consider, or for vets to commonly recommend.  I see it commonly go for 65-105$ per test..just for that ONE TEST!!! Lisa and fur-brats Akira--FeLV miracle baby www.geocities.com/anzajaguar Indy-- Truley Indian Jones as a cat..FEARLESS Lance- Mini wire haired dashchund Bow-Tie- 2yr old (ssshhhh he doesnt know he is 15 :) ) jack russel cross Bennie Bird-- Vampire cockatiel Anza-- sexist Senegal parrot..deffinantly a ladies bird :)