In answer to Michelle's question...

A while back we had someone post on this list who was subsequently "kicked 
off" because his style of expressing himself rubbed some listmembers the wrong 
way.
I thought that was unfortunate because this person has a really awesome 
personal library, is well-read and evidently rubs shoulders with vets and/or 
researchers and might have had much useful information to share with us.  This 
person provided the first, and best, explanation I've found so far to explain 
why 
some cats can live with the virus long term, while other cats develop fatal 
anemia, lymphomas/other types of cancer, which are fatal.
  
It seems that all cats who become infected with the FeLV virus are infected 
with the FeLV-A subgroup.  This manifestation of the virus is the only one that 
is passed from cat to cat, and in the scheme of things is less serious in 
that it causes significant immunosuppression, but not much else.  Provided a 
cat's FeLV infection does not mutate beyond this state, there is a reasonable 
prognosis for long-term survival, even if the infection has progressed to stage 
5, 
at which point the virus has settled in the bone marrow and the cat will thus 
remain infected for the rest of his/her life.  It is in stage 6 that the 
mutation and development of fatal symptoms develops.  An ELISA+ can indicate 
even 
the earlier stages of infection, while an IFA+ correlates with stage 4 on.

Other subgroups of the FeLV virus develop within an individual cat through 
mutation and recombination with that cat's DNA.  The FeLV-A+B subgroup is 
associated with the development of various types of lymphoma or other cancers, 
while 
the FeLV-A+C subgroup is the one associated with severe/nonregenerative 
anemia.  (Within each subgroup there is more than one "strain," but that seems 
to 
be considered useful info only to researchers.)  It is possible for both the B 
and C subgroups to manifest in a particular cat either simultaneously or in 
tandem so that the cat will have FeLV-A, B+C, which I suppose is the worst case 
scenario as a cat with some form of lymphoma or cancer might still be 
considered to have some sort of chance to respond to treatment, but one which 
also has 
nonregenerative anemia and can no longer prodcue red blood cells has no 
chance at all.

It IS possible, in research settings to determine which subgroup(s) of the 
virus a cat may be infected with, but, since there is yet no known way to 
prevent a mutation from occurring or forestall the development of symptoms once 
it 
has, that info is not considered to be useful info except to researchers. It 
becomes obvious when the serious symptoms of lymphoma/cancer, and/or 
nonregenerative anemia present that the infection in a particular cat has 
progressed 
beyond a simple FeLV-A state to one of its more serious manifestations.  Once a 
cat has "crashed" and presents with such symptoms, there is, so far, no known 
treatment that can reliably affect the eventual outcome, which is death.

The wild card in all of this seems to be the relative strength of each 
individual cat's immune response, or whatever immune response he/she still has 
after 
becoming infected with FeLV-A. ( Wouldn't it be great if there were some way 
to test and measure this?!)  In this regard it must be assumed there is a 
genetic component.  Some cats are blessed and initially come into this world 
with 
a stronger immune system than others.  

Another factor is how much and what kinds of stressors a cats's immune system 
may be subjected to, in terms of poor nutrition, other diseases, 
environmental toxins/pollution, the over-use of vaccines, emotional stress and 
deprivation, etc.

Kittens are most at risk, with geriatric cats whose immune function is 
declining at an increased-if-not-high risk.  Most healthy, adult cats are 
thought to 
be naturally and relatively resistant to infection by the FeLV virus and if 
exposed and transiently "infected"  are able to clear the virus even up to 
stage 4 of the infectious process.  Kittens are most at risk because they can 
become infected in utero from an infected mom, or soon after birth through 
nursing.  After weaning, young kittens are still at high risk because their 
immune 
systems are immature and still developing.  For whatever reason, 50 times more 
FeLV virus replicates in the lymphocytes of kittens than in the lymphocytes of 
adult cats.

It is not so unusual, then, for an adult cat to be exposed, become infected 
to the extent an ELISA will yield a positive result, or even an IFA positive if 
the cat is still in stage 4, and for the infection to be only transient and 
the cat able to seroconvert back to negative status.  There is also the 
possibility, however, that the infection could have become latent and later be 
triggered back into active status by vaccination or some other type of stress 
to the 
immune system.

For kittens, however, it is all but unheard of for one who initially tests 
ELISA+ that is IFA confirmed to be able to seroconvert.  Such an instance would 
be highly suspect, with the need to test for a latent infection indicated.

I have not yet come across a valid explanation as to why a kitten/cat might 
initially test negative, then later test positive, with no interim exposure to 
an FeLV+ cat.  I speculate that the infection might have been in the 
incubation process when the test was performed.  Or, is it possible for an 
infection to 
be initially latent??  These are two questions to which I would like to find 
the answer.  

For reasons that I still do not understand, some young kittens initially test 
ELISA+ but later retest negative.  I have one such kitten who at 6 weeks 
tested positive and was going to be PTS if I had not rescued him.  Because of 
the 
cost, I did not have that result confirmed with an IFA, but just assumed he 
would remain positive for however long he lived.  When he was neutered at 5-6 
mos. old, he retested NEGATIVE on an ELISA.  Is he truly a miracle baby who has 
cleared the virus, or has the infection become dormant?  Without doing an 
expensive, invasive and stressful bone marrow reactivation test, it seems I 
won't 
know for sure.  I was hoping that there was some other less invasive test, 
such as the PCR test, which might provide the necessary answer.  But, according 
to Dr. Susan, unless that  test is meticulously performed and interpreted, it 
would not be reliable for that purpose.

It is said that "false negatives" on an ELISA would only be due to error on 
the part of the test administator, so are rare.  A true "false positive" on an 
ELISA would be for FeLV antigen to be detected when none was actually present, 
so that term is a bit of a misnomer.  What is more likely is that because the 
ELISA test is 100 times more sensitive at detecting the antigen than is the 
IFA, a positive result can be obtained when only minute amounts of antigen are 
present and perhaps if a particular kitten is fortunate enough to have a 
particularly strong immune response, and the exposure was not intense, the 
infection might not become permanent.  That, of course, is speculation on my 
part and 
this is an aspect about the testing and infectious process about which I seek 
to learn more.

I had previously (and erroneously) thought such transient positives in 
kittens were because when tested before weaning or soon after, whatever 
antibodies 
were passed from the mom to her kittens were still in play and until the kitten 
was old enough and far enough past weaning for his/her own immune function to 
be starting to mature, it was not possible to get a truly indicative 
reflection of a kitten's FeLV status.  But it has since become clear to me that 
it is 
not antibodies, but the antiGEN produced by the replicating virus that is 
being detected by the ELISA and IFA tests.

So, it would seem that, for now, the best we can do for the FeLV+ kittys who 
find their way to us is to feed them the best possible diet we can -- 
preferably a raw one (Dr. Ihor Basko mentioned in Dr. Goldstein's book claims 
to have 
a good rate of success in that regard and has devised a special diet for FeLV+ 
cats) -- with immunosupportive supplementation, keep stressors in their lives 
to a minimum and protect them from pathogens that might overwhelm a 
compromised immune system.

My limited personal experience, and from reading about the experiences of 
other listmembers in the almost two years I've been a member, is that once a 
cat 
begins to develop and present with major and serious symptoms characteristic 
of the B or C subgroups, it is not a matter of if, but when, the end will come. 
 There seems to be no way to predict which cats will remain in the FeLV-A 
state, and which ones are so ill-fated the virus will mutate into the B, C or 
B+C 
subgroups.  All we can do is provide the best supportive care and diet and 
love them one day at a time.

Sally in San Jose 

I hope and pray that one day it will be understood what it is that causes the 
FeLV virus to mutate from the less severe FeLV-A form to one of its more 
virulent subgrous.  Perhaps then a viable form of treatment can be formulated 
to 
prevent this from occurring and give more kittys a better chance for long-term 
survival.    

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