Thanks very much for the help. Staden is just what I need - in fact it looks excellent and I can't believe I didn't find it earlier! Cheers, David.





On Thursday, December 12, 2002, at 01:23 AM, andy law (RI) wrote:

David,

Staden is what you want/need. Free to academics. Home page is at http://www.mrc-lmb.cam.ac.uk/pubseq. Also a Mac OSX version

HTH

Andy

Dr. Andy Law
--------------------
Head of Bioinformatics - Roslin Institute

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-----Original Message-----
From: David Orlovich [mailto:[EMAIL PROTECTED]]
Sent: 10 December 2002 20:18
To: [EMAIL PROTECTED]
Subject: [Fink-users] DNA sequence viewer


Hello Fink users.

I'm writing to ask if anyone knows of open source software available
for MacOS/X11 that allows the user to view, manipulate and edit
electropherograms from an ABI DNA sequencer.  Specifically,
what I need
is an alternative to proprietary software like Genecodes'
Sequencher or
ABI's AutoAssembler.  There are freeware programs like ABI's
Editview,
that allow the visualisation of single electropherograms, but
I'm been
unable to find free software that will allow the user to assemble and
edit contiguous sequences (contigs).  Typically, most users of DNA
sequencers would sequence forward and reverse strands and
then assemble
the strands to form a consensus sequence, which is then cut
and pasted
into a text file in FASTA format for subsequent processing (multiple
alignment etc).

I've had a bit of a look at some packages available through Fink,
notably Emboss, but this doesn't do what I've described above.  Any
help would be much appreciated.  I think there would be an
overwhelming
demand for such an open source program since most of the downstream
processing can be done for free by quite good software - I just can't
believe that people without access to commercial software resort to
editing electropherograms by hand from printouts of the ABI files to
get the sequences in the first place.

Any thoughts, suggestions, comments?

Cheers, David Orlovich.



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