Hi Fred,

I'll have to defer to Doug on this
Bruce
On Tue, 30 Apr 2013, Fred Dick wrote:

> Hi Bruce
>
>
> On 30 Apr 2013, at 13:36, Bruce Fischl <fis...@nmr.mgh.harvard.edu> wrote:
>
>> Hi Fred
>>
>> I know it's a silly question but are you sure that file exists and is 
>> readable by you? The fact that it's calling GCAread means it's doing the 
>> right thing and support gca files.
>
> Totally not a silly question. With tkregister tho it looks like the path to 
> gca is hard coded somewhere (but not in tkregister.tcl). So after finally 
> engaging my brain  I realized I could just make a symlink to the path 
> tkregister is looking for  (e.g., 
> /usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca) and then 
> it works.
>
> So that's great - and then if I use an --ltaout flag, tkregister helpfully 
> writes out the .lta. Which mysteriously is different than the original 
> talairach.lta.
>
> Is it the inverse transform with an offset, perhaps?
>
> The reason I ask is that it looks like you can tweak non-ideal .lta with 
> tkregister. I think Matt Glasser wrote something related a while back, but 
> not sure.
>
> Thanks as always,
>
> Fred
>
>
>
>
>
>> You can also extra the mean of the highest prob label (-nth 0) and the label 
>> itself (-nth 1) using mri_convert:
>>
>> mri_convert -nth 0 \
>> /usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca \
>> label_means.mgz
>>
>> mri_convert -nth 1 \
>> /usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca \
>> labels.mgz
>>
>>
>> cheers
>> Bruce
>>
>>
>> On Tue, 30 Apr 2013, Fred Dick wrote:
>>
>>> Hi again
>>>
>>> different weighting sorta kinda worked better. It might be a dud brain.
>>>
>>> But on this topic - I realized I wasn't quite sure of a couple of things, 
>>> and had some errors pop up with some tkregister2 options.
>>>
>>> [btw, running stable 5.2.0 on snow leopard]
>>>
>>> 1) I first tried to check the reg to the gca, and got the following error 
>>> message. It looks like maybe this is an old/deprecated option given the 
>>> hard-coded path to the 5.0.0 linux version (I saw a posting from Doug from 
>>> a few years ago on this)
>>>
>>>
>>> ------------------------------------------------------------------------------------------------------------------------------------------
>>> [rancate:/Applications/freesurfer/subjects] fred% tkregister2 --check-reg 
>>> --gca dancar
>>> tkregister_tcl /Applications/freesurfer/tktools/tkregister2.tcl
>>> INFO: LTA input is not RAS to RAS...converting...
>>> target  volume /Applications/freesurfer/subjects/dancar/mri/T1.mgz
>>> movable 
>>> volume/usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca
>>> reg file       /tmp/reg.tmp.1367946635.dat
>>> LoadVol        1
>>> ZeroCRAS       0
>>> $Id: tkregister2.c,v 1.121.2.1 2011/03/28 20:25:16 greve Exp $
>>> Diagnostic Level -1
>>> GCAread(/usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca):
>>>  could not open file
>>> No such file or directory
>>> ERROR: could not read 
>>> /usr/local/freesurfer-5.0.0-cent4-64/average/RB_all_2008-03-26.gca as 22
>>> ------------------------------------------------------------------------------------------------------------------------------------------
>>>
>>> 2) I also tried to load up the gca in tkmedit using the appropriate gui 
>>> option, but nothing happened (no error messages either)
>>>
>>> 3) Then tried another trick that Doug suggested a while ago on the message 
>>> boards:
>>>
>>> tkregister2 --mov $FREESURFER_HOME/average/RB_all_2008-03-26.gca --targ 
>>> $SUBJECTS_DIR/dancar/mri/brain.mgz --lta-inv 
>>> $SUBJECTS_DIR/dancar/mri/transforms/talairach.lta --check-reg
>>>
>>> and got "ERROR: Option --lta-inv unknown"
>>>
>>> 4) I finally tried using the talairach.lta as a transform to the fsl 
>>> target, using some old default-processed brains to make sure it was 
>>> correct-ish:
>>>
>>> tkregister2 --check-reg --lta transforms/talairach.lta --mov ./T1.mgz 
>>> --fsl-targ
>>>
>>> This seemed in the ballpark - except when I looked at bert, who I assumed 
>>> would be canonical, and transform was clearly not correct. (Given Doug's 
>>> earlier command, it seemed like talairach.lta was the inverse transform of 
>>> what I thought it was, e.g., sub => standard).
>>>
>>> So I'm slightly stymied.
>>>
>>> Thanks tons,
>>>
>>> Fred
>>>
>>>
>>>
>>>
>>> On 29 Apr 2013, at 15:06, Fred Dick <ubjt...@mail.bbk.ac.uk> wrote:
>>>
>>>> Aha, I shall quickly try with the non -w weighting and see what happens. 
>>>> Thanks!
>>>> On 29 Apr 2013, at 15:05, Bruce Fischl <fis...@nmr.mgh.harvard.edu> wrote:
>>>>
>>>>> yes, the -w will create an optimal weighting to maximize gray/white 
>>>>> contrast. The image it generates won't in general be physically 
>>>>> realizable in an real MR acquisition. Not surprising that the tal stuff 
>>>>> fails
>>>>> On Mon, 29 Apr 2013, Fred Dick wrote:
>>>>>
>>>>>> Hi Bruce
>>>>>>
>>>>>> I'm using TR=20, flip=30, TE=5 - but possibly irrelevant as also have 
>>>>>> the -w flag on.
>>>>>>
>>>>>> I originally thought this was from a bad brainmask, but then generated a 
>>>>>> good one and same thing happening.
>>>>>>
>>>>>>
>>>>>> On 29 Apr 2013, at 14:27, Bruce Fischl <fis...@nmr.mgh.harvard.edu> 
>>>>>> wrote:
>>>>>>
>>>>>>> Hi Fred
>>>>>>>
>>>>>>> we don't have much experience with this. Which synthesis are you using? 
>>>>>>> You might need to use a different one for the talairaching to more 
>>>>>>> closely match the atlas (maybe a TR=20,TE=2.5,flip=20-30 one)
>>>>>>>
>>>>>>>
>>>>>>> On Mon, 29 Apr 2013, Fred Dick wrote:
>>>>>>>
>>>>>>>> Dear all
>>>>>>>>
>>>>>>>> I'm having difficulties getting correct Talairach.lta's from synthetic 
>>>>>>>> flash volumes (Bruce, you are probably sorry you ever told me about 
>>>>>>>> this!).  Basically the brain is over-scaled (bigger than it should 
>>>>>>>> be), which I think is happening because the contrast is so good (gm 
>>>>>>>> much darker than wm).
>>>>>>>>
>>>>>>>> Is there an example of how to use the -flash_parms flag, and what 
>>>>>>>> mri_em_register is expecting in the parameter file?
>>>>>>>>
>>>>>>>> Thanks,
>>>>>>>>
>>>>>>>> Fred
>>>>>>>>
>>>>>>>>
>>>>>>>>
>>>>>>>>
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>>>>>>>>
>>>>>>>>
>>>>>>>>
>>>>>>>
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>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>
>>>
>>>
>>>
>>>
>
>
>
>
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