>
> Hi Doug and all,
>

    I just resend this email in case it did not reach, any help will be
appreciated.

>
> Thanks for your help. I was using the preprocessed thinking that after
> motion correction it would be more accurate and --init-header because they
> where acquired in the same session. I changed to a raw moveable and the
> results did not change for the following:
>
> With -init-fsl the moveable is now showing, but about 40% of the subjects
> the registration has fail, in many cases the moveable is rotated with
> respect to the target. I tried to use -init-spm but then the moveable and
> target don't even overlay.
>
> I also tried to use a successful registration .dat from another subject
> using the command  --init-reg $SUBJECTS_DIR/subj2/mri/register.dat  but
> the registration is not accurate.
>
> In many cases there is the WARNING: initial G-W contrast was negative, but
> expected positive. If the mov data has a T1 contrast, re-run with --T1. if
> I run the same command changing --bold to --T1 the results aren't good, as
> well as if I try -int $SUBJECTS_DIR/subj//T1.mgz.
>
> Is there anything else to try rather than manual registration? I just have
> the T1 and the fmri.  Would you recommend another registration method?
>
> Thanks
> Adonay
>
>
> On Mon, Sep 12, 2016 at 12:37 PM, Douglas N Greve <
> gr...@nmr.mgh.harvard.edu> wrote:
>
>> I think the problem is the initialization. I noticed you used
>> --init-header. This should only be used if the two volumes were acquired
>> during the same session and the direction cosines (ie, geometry) info is
>> accurate for both. Is this the case? I noticed that the input has
>> "preproc" in the name, so I'm a bit suspicious. You can try --init-fsl
>> instead
>>
>>
>> On 09/12/2016 01:36 PM, A Nunes wrote:
>> > Hi Doug
>> >
>> > I can't see any errors in the log file, and with other datasets I did
>> > not have this issue. My register.dat is:
>> >
>> > 50664
>> > 3.000000
>> > 4.500000
>> > 0.150000
>> > 9.996076822280884e-01 -2.117119915783405e-02 -1.833262108266354e-02
>> > 3.139470825195312e+02
>> > 1.872027479112148e-02 1.825972087681293e-02 9.996579289436340e-01
>> > 2.150849456787109e+02
>> > -2.082921005785465e-02 -9.996089935302734e-01 1.864889077842236e-02
>> > 4.339286499023438e+02
>> > 0 0 0 1
>> > round
>> >
>> > and my register.dat.log is:
>> >
>> > setenv SUBJECTS_DIR /Users/adonay/Desktop/data/freesurfer/5
>> > cd /Users/adonay/Desktop/data/prepo_cpac
>> > /Applications/freesurfer/bin/bbregister --s 50664 --mov
>> > 50664_preprocessed.nii.gz --init-header --reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat --bold
>> >
>> > $Id: bbregister,v 1.49.2.3 2013/03/25 18:04:53 greve Exp $
>> > Darwin sc-155014 15.6.0 Darwin Kernel Version 15.6.0: Mon Aug 29
>> > 20:21:34 PDT 2016; root:xnu-3248.60.11~1/RELEASE_X86_64 x86_64
>> > FREESURFER_HOME /Applications/freesurfer
>> > mri_convert 50664_preprocessed.nii.gz
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > mri_convert 50664_preprocessed.nii.gz
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> >
>> > $Id: mri_convert.c,v 1.179.2.7 2012/09/05 21:55:16 mreuter Exp $
>> > reading from 50664_preprocessed.nii.gz...
>> > TR=2000.00, TE=0.00, TI=0.00, flip angle=0.00
>> > i_ras = (-1, -0, 0)
>> > j_ras = (-0, 1, 0)
>> > k_ras = (-0, -0, 1)
>> > writing to
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii...
>> > tkregister2_cmdl --s 50664 --mov
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > --regheader --reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.init.dat
>> > --noedit
>> > tkregister_tcl /Applications/freesurfer/tktools/tkregister2.tcl
>> > INFO: no target volume specified, assuming FreeSurfer orig volume.
>> > target  volume orig
>> > movable volume
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > reg file
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.init.dat
>> > LoadVol        0
>> > ZeroCRAS       0
>> > $Id: tkregister2.c,v 1.121.2.1 2011/03/28 20:25:16 greve Exp $
>> > Diagnostic Level -1
>> > INFO: loading target
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/orig.mgz
>> > Ttarg: --------------------
>> > -1.000   0.000   0.000   128.000;
>> >  0.000   0.000   1.000  -128.000;
>> >  0.000  -1.000   0.000   128.000;
>> >  0.000   0.000   0.000   1.000;
>> > INFO: loading movable
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > Tmov: --------------------
>> > -3.000   0.000   0.000   96.000;
>> >  0.000   0.000   4.500  -63.000;
>> >  0.000  -3.000   0.000   96.000;
>> >  0.000   0.000   0.000   1.000;
>> > mkheaderreg = 1, float2int = 0
>> > Computing reg from header (and possibly input matrix)
>> > ---- Input registration matrix (computed) --------
>> >  1.000   0.000   0.000   307.685;
>> >  0.000   0.000   1.000   216.216;
>> >  0.000  -1.000   0.000   435.341;
>> >  0.000   0.000   0.000   1.000;
>> > ---------------------------------------
>> > ---- Input registration matrix --------
>> >  1.000   0.000   0.000   307.685;
>> >  0.000   0.000   1.000   216.216;
>> >  0.000  -1.000   0.000   435.341;
>> >  0.000   0.000   0.000   1.000;
>> > Determinant 1
>> > subject = 50664
>> > RegMat ---------------------------
>> >  1.000   0.000   0.000   307.685;
>> >  0.000   0.000   1.000   216.216;
>> >  0.000  -1.000   0.000   435.341;
>> >  0.000   0.000   0.000   1.000;
>> > mri_segreg --mov
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > --init-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.init.dat
>> > --out-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > --subsamp-brute 100 --subsamp 100 --tol 1e-4 --tol1d 1e-3 --brute -4 4
>> > 4 --surf white --gm-proj-frac 0.5 --gm-gt-wm 0.5
>> > $Id: mri_segreg.c,v 1.103.2.1 2011/10/07 19:14:42 greve Exp $
>> > setenv SUBJECTS_DIR /Users/adonay/Desktop/data/freesurfer/5
>> > cd /Users/adonay/Desktop/data/prepo_cpac
>> > mri_segreg --mov
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > --init-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.init.dat
>> > --out-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > --subsamp-brute 100 --subsamp 100 --tol 1e-4 --tol1d 1e-3 --brute -4 4
>> > 4 --surf white --gm-proj-frac 0.5 --gm-gt-wm 0.5
>> > sysname  Darwin
>> > hostname sc-155014
>> > machine  x86_64
>> > user     adonay
>> > movvol
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > regfile
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.init.dat
>> > subject 50664
>> > dof 6
>> > outregfile
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > UseMask 0
>> > UseLH 1
>> > UseRH 1
>> > nsubsamp 100
>> > PenaltySign  -1
>> > PenaltySlope 0.500000
>> > PenaltyCenter 0.000000
>> > surfname white
>> > GMProjFrac 0.500000
>> > WMProjAbs 2.000000
>> > lhcostfile (null)
>> > rhcostfile (null)
>> > interp  trilinear (1)
>> > frame  0
>> > TolPowell 0.000100
>> > nMaxItersPowell 36
>> > n1dmin  3
>> > Profile   0
>> > Gdiag_no  -1
>> > AddNoise  0 (0)
>> > SynthSeed 1473407392
>> > TransRandMax 0.000000
>> > RotRandMax 0.000000
>> > Translations 0.000000 0.000000 0.000000
>> > Rotations   0.000000 0.000000 0.000000
>> > Input reg
>> >  1.000   0.000   0.000   307.685;
>> >  0.000   0.000   1.000   216.216;
>> >  0.000  -1.000   0.000   435.341;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Loading mov
>> > Extracting frame 0
>> > Projecting LH Surfs
>> > Loading lh.white surf
>> > Loading lh.thickness for GM
>> > GM Proj: 1 0.500000 2.000000
>> > WM Proj: 0 0.500000 2.000000
>> > Projecting RH Surfs
>> > Loading rh.white surf
>> > Loading rh.thickness
>> > Projecting RH Surfs
>> > Computing relative cost
>> >  0  -25.0 -25.0 -25.0   10.000000
>> >  1  -25.0 -25.0  25.0   10.000000
>> >  2  -25.0  25.0 -25.0   10.000000
>> >  3  -25.0  25.0  25.0   10.000000
>> >  4   25.0 -25.0 -25.0   10.000000
>> >  5   25.0 -25.0  25.0   10.000000
>> >  6   25.0  25.0 -25.0   10.000000
>> >  7   25.0  25.0  25.0   10.000000
>> > REL:  8  10.000000   80.000000  10.000000 rel = 1
>> > Initial costs ----------------
>> > Number of surface hits 0
>> > WM  Intensity        nan +/-      nan
>> > Ctx Intensity        nan +/-      nan
>> > Pct Contrast         nan +/-      nan
>> > Cost  10.0000
>> > RelCost   1.0000
>> >
>> > ------------------------------------
>> > Brute force preopt -4 4 4, n = 729
>> >      0  -4.0000  -4.0000  -4.0000  -4.0000  -4.0000  -4.0000
>> > 10.0000  10.0000  0.0
>> > Brute Force --------------------------
>> > Min cost was 10.000000
>> > Number of iterations   729
>> > Search time 0.723000 sec
>> > Parameters at best (transmm, rotdeg)
>> >  -4.000  -4.000  -4.000 -4.000 -4.000 -4.000
>> > --------------------------------------------
>> >
>> > Starting Powell Minimization
>> > Init Powel Params dof = 6
>> > 0 -4
>> > 1 -4
>> > 2 -4
>> > 3 -4
>> > 4 -4
>> > 5 -4
>> > Powell done niters = 0
>> > Computing relative cost
>> >  0  -25.0 -25.0 -25.0   10.000000
>> >  1  -25.0 -25.0  25.0   10.000000
>> >  2  -25.0  25.0 -25.0   10.000000
>> >  3  -25.0  25.0  25.0   10.000000
>> >  4   25.0 -25.0 -25.0   10.000000
>> >  5   25.0 -25.0  25.0   10.000000
>> >  6   25.0  25.0 -25.0   10.000000
>> >  7   25.0  25.0  25.0   10.000000
>> > REL:  8  10.000000   80.000000  10.000000 rel = 1
>> > Number of iterations     0
>> > Min cost was 10.000000
>> > Number of FunctionCalls   109
>> > TolPowell 0.000100
>> > nMaxItersPowell 36
>> > OptimizationTime 0.105000 sec
>> > Parameters at optimum (transmm) -1.38389 -1.38389 -1.38389
>> > Parameters at optimum (rotdeg) -1.38389 -1.38389 -1.38389
>> > Final costs ----------------
>> > Number of surface hits 0
>> > WM  Intensity        nan +/-      nan
>> > Ctx Intensity        nan +/-      nan
>> > Pct Contrast         nan +/-      nan
>> > Cost  10.0000
>> > RelCost   1.0000
>> > Reg at min cost was
>> >  0.999   0.024   0.025   301.214;
>> > -0.024  -0.025   0.999   218.039;
>> >  0.024  -0.999  -0.024   435.913;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Writing optimal reg to
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat,
>> > type = 14
>> > Original Reg
>> >  1.000   0.000   0.000   307.685;
>> >  0.000   0.000   1.000   216.216;
>> >  0.000  -1.000   0.000   435.341;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Original Reg - Optimal Reg
>> >  0.001  -0.024  -0.025   6.471;
>> >  0.024   0.025   0.001  -1.823;
>> > -0.024  -0.001   0.024  -0.573;
>> >  0.000   0.000   0.000   0.000;
>> >
>> > Computing change in lh position
>> > LH rmsDiffMean 6.969701
>> > Computing change in rh position
>> > Surface RMS Diff (mm) 6.712317 9.940387
>> > mri_segreg done
>> > mri_segreg --mov
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > --init-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > --out-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat --brute
>> > -0.1 0.1 0.1 --interp trilinear --wm-proj-abs 2 --tol 1e-8 --tol1d
>> > 1e-3 --c0 0 --mincost
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat.mincost
>> > --dof 6 --nmax 36 --param
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat.param
>> > --surf white --cur-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.curopt.dat
>> > --gm-proj-frac 0.5 --nsub 1 --gm-gt-wm 0.5
>> > $Id: mri_segreg.c,v 1.103.2.1 2011/10/07 19:14:42 greve Exp $
>> > setenv SUBJECTS_DIR /Users/adonay/Desktop/data/freesurfer/5
>> > cd /Users/adonay/Desktop/data/prepo_cpac
>> > mri_segreg --mov
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > --init-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > --out-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat --brute
>> > -0.1 0.1 0.1 --interp trilinear --wm-proj-abs 2 --tol 1e-8 --tol1d
>> > 1e-3 --c0 0 --mincost
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat.mincost
>> > --dof 6 --nmax 36 --param
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat.param
>> > --surf white --cur-reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/reg.curopt.dat
>> > --gm-proj-frac 0.5 --nsub 1 --gm-gt-wm 0.5
>> > sysname  Darwin
>> > hostname sc-155014
>> > machine  x86_64
>> > user     adonay
>> > movvol
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/template.nii
>> > regfile
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/tmp.bbregi
>> ster.14726/bbr.pass1.dat
>> > subject 50664
>> > dof 6
>> > outregfile /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.
>> dat
>> > UseMask 0
>> > UseLH 1
>> > UseRH 1
>> > nsubsamp 1
>> > PenaltySign  -1
>> > PenaltySlope 0.500000
>> > PenaltyCenter 0.000000
>> > surfname white
>> > GMProjFrac 0.500000
>> > WMProjAbs 2.000000
>> > lhcostfile (null)
>> > rhcostfile (null)
>> > interp  trilinear (1)
>> > frame  0
>> > TolPowell 0.000000
>> > nMaxItersPowell 36
>> > n1dmin  3
>> > Profile   0
>> > Gdiag_no  -1
>> > AddNoise  0 (0)
>> > SynthSeed 1474349903
>> > TransRandMax 0.000000
>> > RotRandMax 0.000000
>> > Translations 0.000000 0.000000 0.000000
>> > Rotations   0.000000 0.000000 0.000000
>> > Input reg
>> >  0.999   0.024   0.025   301.214;
>> > -0.024  -0.025   0.999   218.039;
>> >  0.024  -0.999  -0.024   435.913;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Loading mov
>> > Extracting frame 0
>> > Projecting LH Surfs
>> > Loading lh.white surf
>> > Loading lh.thickness for GM
>> > GM Proj: 1 0.500000 2.000000
>> > WM Proj: 0 0.500000 2.000000
>> > Projecting RH Surfs
>> > Loading rh.white surf
>> > Loading rh.thickness
>> > Projecting RH Surfs
>> > Computing relative cost
>> >  0  -25.0 -25.0 -25.0   10.000000
>> >  1  -25.0 -25.0  25.0   10.000000
>> >  2  -25.0  25.0 -25.0   10.000000
>> >  3  -25.0  25.0  25.0   10.000000
>> >  4   25.0 -25.0 -25.0   10.000000
>> >  5   25.0 -25.0  25.0   10.000000
>> >  6   25.0  25.0 -25.0   10.000000
>> >  7   25.0  25.0  25.0   10.000000
>> > REL:  8  10.000000   80.000000  10.000000 rel = 1
>> > Initial costs ----------------
>> > Number of surface hits 0
>> > WM  Intensity        nan +/-      nan
>> > Ctx Intensity        nan +/-      nan
>> > Pct Contrast         nan +/-      nan
>> > Cost  10.0000
>> > RelCost   1.0000
>> >
>> > ------------------------------------
>> > Brute force preopt -0.1 0.1 0.1, n = 729
>> >      0  -0.1000  -0.1000  -0.1000  -0.1000  -0.1000  -0.1000
>> > 10.0000  10.0000  0.0
>> > Brute Force --------------------------
>> > Min cost was 10.000000
>> > Number of iterations   729
>> > Search time 0.976000 sec
>> > Parameters at best (transmm, rotdeg)
>> >  -0.100  -0.100  -0.100 -0.100 -0.100 -0.100
>> > --------------------------------------------
>> >
>> > Starting Powell Minimization
>> > Init Powel Params dof = 6
>> > 0 -0.1
>> > 1 -0.1
>> > 2 -0.1
>> > 3 -0.1
>> > 4 -0.1
>> > 5 -0.1
>> > Powell done niters = 0
>> > Computing relative cost
>> >  0  -25.0 -25.0 -25.0   10.000000
>> >  1  -25.0 -25.0  25.0   10.000000
>> >  2  -25.0  25.0 -25.0   10.000000
>> >  3  -25.0  25.0  25.0   10.000000
>> >  4   25.0 -25.0 -25.0   10.000000
>> >  5   25.0 -25.0  25.0   10.000000
>> >  6   25.0  25.0 -25.0   10.000000
>> >  7   25.0  25.0  25.0   10.000000
>> > REL:  8  10.000000   80.000000  10.000000 rel = 1
>> > Number of iterations     0
>> > Min cost was 10.000000
>> > Number of FunctionCalls   109
>> > TolPowell 0.000000
>> > nMaxItersPowell 36
>> > OptimizationTime 3.303000 sec
>> > Parameters at optimum (transmm)  2.51611  2.51611  2.51611
>> > Parameters at optimum (rotdeg)  2.51611  2.51611  2.51611
>> > Final costs ----------------
>> > Number of surface hits 0
>> > WM  Intensity        nan +/-      nan
>> > Ctx Intensity        nan +/-      nan
>> > Pct Contrast         nan +/-      nan
>> > Cost  10.0000
>> > RelCost   1.0000
>> > Reg at min cost was
>> >  1.000  -0.021  -0.018   313.947;
>> >  0.019   0.018   1.000   215.085;
>> > -0.021  -1.000   0.019   433.929;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Writing optimal reg to
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat, type =
>> 14
>> > Original Reg
>> >  0.999   0.024   0.025   301.214;
>> > -0.024  -0.025   0.999   218.039;
>> >  0.024  -0.999  -0.024   435.913;
>> >  0.000   0.000   0.000   1.000;
>> >
>> > Original Reg - Optimal Reg
>> > -0.000   0.045   0.043  -12.733;
>> > -0.043  -0.043  -0.000   2.954;
>> >  0.045   0.000  -0.043   1.985;
>> >  0.000   0.000   0.000   0.000;
>> >
>> > Computing change in lh position
>> > LH rmsDiffMean 13.402319
>> > Computing change in rh position
>> > Surface RMS Diff (mm) 13.076895 18.900088
>> > mri_segreg done
>> > Cleaning up
>> > Started at Thu Sep 8 19:09:02 PDT 2016
>> > Ended   at Thu Sep  8 19:09:28 PDT 2016
>> > BBR-Run-Time-Sec 26
>> > bbregister Done
>> > To check results, run:
>> > tkregister2 --mov 50664_preprocessed.nii.gz --reg
>> > /Users/adonay/Desktop/data/freesurfer/5/50664/mri/register.dat --surf
>> >
>> >
>> >
>> >
>> >
>> > Thanks,
>> > Adonay
>> >
>> > Douglas Greve
>> > <http://www.mail-archive.com/search?l=freesur...@nmr.mgh.har
>> vard.edu&q=from:%22Douglas+Greve%22>
>> > Sun, 11 Sep 2016 19:14:45 -0700
>> > <http://www.mail-archive.com/search?l=freesur...@nmr.mgh.har
>> vard.edu&q=date:20160911>
>> >
>> > What is your command line and log file? Sounds like the initial
>> > registration failed.
>> >
>> >
>> > ---------- Forwarded message ----------
>> > From: *A Nunes* <adonay.s.nu...@gmail.com
>> > <mailto:adonay.s.nu...@gmail.com>>
>> > Date: Sun, Sep 11, 2016 at 7:04 PM
>> > Subject: Tkregister2 error, moveable not present just white screen
>> > To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu
>> > <mailto:freesurfer@nmr.mgh.harvard.edu>>
>> >
>> >
>> > Hi,
>> >
>> > After doing a bbregistration I visualized the results with
>> > tkregister2. The target is alright, however, the moveable is not
>> > present, it is just a white screen.
>> >
>> > What steps could I take to solve this issue?
>> >
>> > Thanks
>> > Adonay
>> >
>> >
>> >
>> > _______________________________________________
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>>
>> --
>> Douglas N. Greve, Ph.D.
>> MGH-NMR Center
>> gr...@nmr.mgh.harvard.edu
>> Phone Number: 617-724-2358
>> Fax: 617-726-7422
>>
>> Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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>> www.nmr.mgh.harvard.edu/facility/filedrop/index.html
>> Outgoing: ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
>>
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