External Email - Use Caution Hello Freesurfer experts & Eugenio,
I have been trying to extract the volumes of hippocampal subfields using the longitudinal pipeline ( https://surfer.nmr.mgh.harvard.edu/fswiki/LongitudinalHippocampalSubfields) on macOS Mojave using freesurfer-Darwin-OSX-stable-pub-v6.0.0-2beb96c. For some reason, both the lh.hippoSfVolumes-T1.long.v10.txt and rh.hippoSfVolumes-T1.long.v10.txt text files show negative volumes as shown below. Hippocampal_tail -445.418232 subiculum -339.909193 CA1 -559.134087 hippocampal-fissure -165.590803 presubiculum -263.670386 parasubiculum -55.654556 molecular_layer_HP -541.803219 GC-ML-DG -293.794963 CA3 -222.042205 CA4 -266.645751 fimbria -66.106600 HATA -53.397592 Whole_hippocampus -3107.576783 I have also tried to extract the hippocampal subfield volumes from the longitudinally processed standard MNI T1 brain template (MNI152_T1_1mm_brain.nii.gz). And i still encounter the same problem with negative volumes. I could not find the suggested solution for this problem in the freesurfer mailing list. Could you please guide us on this issue? Thank you for your time and support. Best wishes Jay
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