Hi All,

I submitted a job to convert sam to bam, and the job was running forever 
without outputing the result. I then checked the log, and it read:
Traceback (most recent call last):
  File "/mnt/galaxyTools/galaxy-dist/lib/galaxy/jobs/runners/drmaa.py", line 
336, in finish_job
    drm_job_state.job_wrapper.finish( stdout, stderr )
  File "/mnt/galaxyTools/galaxy-dist/lib/galaxy/jobs/__init__.py", line 637, in 
    dataset.set_meta( overwrite = False )
  File "/mnt/galaxyTools/galaxy-dist/lib/galaxy/model/__init__.py", line 875, 
in set_meta
    return self.datatype.set_meta( self, **kwd )
  File "/mnt/galaxyTools/galaxy-dist/lib/galaxy/datatypes/binary.py", line 179, 
in set_meta
    raise Exception, "Error Setting BAM Metadata: %s" % stderr
Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

It means that the samtools is not in the PATH. I tried to set the PATH in a 
couple of methods according the Galaxy documentation:
1. put the path in the env.sh in the tool directory and symbolink default to 
the tool directory, e.g. default -> =/mnt/galaxyTools/tools/samtools/0.1.18 
2. put -v PATH=/mnt/galaxyTools/tools/samtools/0.1.18 in ~/.sge_request
3. put -v PATH=/mnt/galaxyTools/tools/samtools/0.1.18 in /path/sge_request

none of them worked, and I got the above same problem.

Then I checked the job log file in the job_working_directory, and it read:
Samtools Version: 0.1.18 (r982:295)
SAM file converted to BAM

which shows that sge knows the PATH of samtools. To double check it, I added 
samtools index to Galaxy, and it worked well. I am very confused why SGE knows 
the tool path but cannot run the job correctly.

The system I am using is ubuntu on EC2. I checked out the code from galaxy-dist 
on bitbucket. Other tools such as bwa and bowtie worked well using the same 
setting method(put env.sh in the tools directory to set the tool path)

Thank you very much for any help or hints.


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