Jen,

I have also shared history with you File 27 and 32 are fetching empty seq
file. I think since bacterial genome is not having any Chr that may be the
problem, I tried all option just coordinates; Chr1 however the out put is
empty.

Thanks

Kanwar

On Wed, Dec 19, 2012 at 10:07 AM, Jennifer Jackson <j...@bx.psu.edu> wrote:

>  Hello,
>
> Both datasets can be loaded and the "custom reference genome" option used
> with the tool 'Fetch Sequences -> Extract Genomic DNA". Details about
> custom genomes are grouped here in our:
> http://wiki.galaxyproject.org/Support#Custom_reference_genome
>
> To be specific, on the "Extract" tool form, you will use the option:
> "Source for Genomic Data:" as  "History", then for the new menu option
> "Using reference file:", select the fasta dataset of your genome from your
> active history.
>
> If you have trouble, be sure to double check that your formats match those
> required by the tool (listed on tool's form). Detailed custom genome
> troubleshooting help is in the wiki above and file format troubleshooting
> help is here, including links to data specifications:
> http://wiki.galaxyproject.org/Support#Troubleshooting_tool_errors
> (start here, more help in following sections, same wiki)
>
> Best,
>
> Jen
> Galaxy team
>
>
> On 12/19/12 8:19 AM, shamsher jagat wrote:
>
> I have a bacterial genome from ncbi and woulld like to extract seq from
> the corresponding fasta file of bacterial genome. Since i have list of
> coordinates so would not be possible to extract one by one. Is there any
> interface within galxy that i can use.
>
>  Thanks
>
>
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> --
> Jennifer Jacksonhttp://galaxyproject.org
>
>
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