Elwood, It's not clear what steps you're having problems with, so it's difficult to offer help. Here's the outline that I use to RNA-seq running with Galaxy:
*download Samtools, Bowtie2, Tophat2, and the Cufflinks tool suite and add them to my galaxy user's path; *download the iGenomes data package for your genomes: http://cufflinks.cbcb.umd.edu/igenomes.html *configure Galaxy to point to particular data files in iGenomes—specifically, add entries to bowtie2_indices.loc and sam_fa_indices.loc This page will help with this last step: http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup Let us know if you run into problems. Best, J. On Jun 14, 2013, at 10:31 AM, Elwood Linney wrote: > Hello again, > I guess I now realize why there is so much traffic on the user list about > tools, and I also can guess that while the online directions for dealing with > them are written well for those individuals familiar with administration a > server or doing full time bioinformatics, but there are some of us computer > numbskulls that need something like a Galaxy transfer booklet for computer > idiots (biologists) to transfer what we want into a single user or single lab > setup. > I am hoping there is someone out there like myself who worked through the > problem of transferring the Trapnell et al series of programs into a Mac Pro > desktop, namely > NGS: RNA Analysis > 1) Tophat for Illumina > tophat (1.3.3-1.4.0) with bowtie + bowtie indexes > 2) Cufflinks > cufflinks (1.3.0-2.1.1) > 3) Cuffcompare > cufflinks (1.3.0-2.1.1) > 4) Cuffmerge > cuffmerge (1.3.0-2.1.1) > 5) Cuffdiff > cuffdiff (2.1.0-2.1.1) > I have transferred the galaxy framework in a work/galaxy-dist pathway unto > a solid state drive of a 64gb ram mac that has 3 other 2tb drives and have > gotten some valuable advice for transferring large datasets from the same > computer via admin. For the next year at least my needs with be just the RNA > analysis tools above. > Any help will be greatly appreciated. > My intention here is remove my contribution to the traffic on the online > Galaxy, so some people should consider it a worthy cause > > Sincerely, > Elwood Linney > Professor of Molecular Genetics and Microbiology > Duke University Medical Cener > > ___________________________________________________________ > Please keep all replies on the list by using "reply all" > in your mail client. To manage your subscriptions to this > and other Galaxy lists, please use the interface at: > http://lists.bx.psu.edu/ > > To search Galaxy mailing lists use the unified search at: > http://galaxyproject.org/search/mailinglists/
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
