Hi Marius

Ok, nice, it works.
My bad, samtools was not really in the path at galaxy startup.

The job is fine, but I still see these messages :

galaxy.model.metadata DEBUG 2018-01-17 16:37:34,295 setting metadata externally 
failed for HistoryDatasetAssociation 11543: 'invalidated'
galaxy.jobs INFO 2018-01-17 16:38:03,242 Collecting metrics for Job 7068
galaxy.jobs DEBUG 2018-01-17 16:38:03,262 job 7068 ended (finish() executed in 
(29086.817 ms))
galaxy.model.metadata DEBUG 2018-01-17 16:38:03,267 Cleaning up external 
metadata files
galaxy.model.metadata DEBUG 2018-01-17 16:38:03,317 Failed to cleanup 
MetadataTempFile temp files from 
/softs/bioinfo/galaxy-prod/database/job_working_directory/007/7068/metadata_out_HistoryDatasetAssociation_11543_s8UGmV:
 No JSON object could be decoded

Fred

De : Marius van den Beek [mailto:[email protected]]
Envoyé : mercredi 17 janvier 2018 15:33
À : SAPET, Frederic <[email protected]>
Cc : Galaxy-dev <[email protected]>
Objet : Re: [galaxy-dev] Error at the end of a BWA job

Hi Frederic,

samtools couldn't be found, which is required for creating the bam index files
during the metadata setting.
You can install it globally or in the admin panel -> Manage tool dependencies 
-> Find the "Set External Metadata" tool
and hit "Install checked dependencies via Conda".

Best,
Marius

On 17 January 2018 at 15:23, SAPET, Frederic 
<[email protected]<mailto:[email protected]>> wrote:
Hello

I’ve just installed BWA ((Galaxy Version 0.7.17.3) , Galaxy itself is On branch 
release_17.09).

The first run is not a success.

I can see that the BAM is created, but job is tagged as fail (Unable to finish 
job).

It looks like close to this issue: 
https://github.com/galaxyproject/galaxy/issues/2083
I’ve checked, samtools is in the path.

Do you have any ideas ?

Thank you

Fred

Here is what I see in the log:

galaxy.jobs.runners.drmaa DEBUG 2018-01-17 10:57:23,787 (7062/86331.universe) 
state change: job finished normally
galaxy.model.metadata DEBUG 2018-01-17 10:57:23,993 setting metadata externally 
failed for HistoryDatasetAssociation 11537: 'invalidated'
galaxy.jobs.runners ERROR 2018-01-17 10:57:24,108 (7062/86331.universe) Job 
wrapper finish method failed
Traceback (most recent call last):
  File "/softs/bioinfo/galaxy-prod/lib/galaxy/jobs/runners/__init__.py", line 
630, in finish_job
    job_state.job_wrapper.finish(stdout, stderr, exit_code)
  File "/softs/bioinfo/galaxy-prod/lib/galaxy/jobs/__init__.py", line 1287, in 
finish
    dataset.datatype.set_meta(dataset, overwrite=False)
  File "/softs/bioinfo/galaxy-prod/lib/galaxy/datatypes/binary.py", line 424, 
in set_meta
    exit_code = subprocess.call(args=command, stderr=open(stderr_name, 'wb'))
  File "/softs/add-ons/Python-2.7.12/lib/python2.7/subprocess.py", line 523, in 
call
    return Popen(*popenargs, **kwargs).wait()
  File "/softs/add-ons/Python-2.7.12/lib/python2.7/subprocess.py", line 711, in 
__init__
    errread, errwrite)
  File "/softs/add-ons/Python-2.7.12/lib/python2.7/subprocess.py", line 1343, 
in _execute_child
    raise child_exception
OSError: [Errno 2] No such file or directory

---
Frederic Sapet
Bioinformatics Research Engineer
BIOGEMMA - Upstream Genomics Group
Centre de Recherche de Chappes
CS 90126
63720 CHAPPES
FRANCE
Tel : +33 (0)4 73 67 88 54<tel:+33%204%2073%2067%2088%2054>
Fax : +33 (0)4 73 67 88 99<tel:+33%204%2073%2067%2088%2099>
E-mail : [email protected]<mailto:[email protected]>


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